Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31725 | 3' | -55.9 | NC_006938.1 | + | 17768 | 0.66 | 0.737622 |
Target: 5'- ---cGUGGCACggGCCUCGgCCUUGuUGc -3' miRNA: 3'- gcuaCAUCGUG--CGGAGCgGGAGCuAC- -5' |
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31725 | 3' | -55.9 | NC_006938.1 | + | 1377 | 0.66 | 0.737622 |
Target: 5'- -uGUGUAGCGCGCCgUGCCgugugugaUUCGAa- -3' miRNA: 3'- gcUACAUCGUGCGGaGCGG--------GAGCUac -5' |
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31725 | 3' | -55.9 | NC_006938.1 | + | 54585 | 0.66 | 0.737622 |
Target: 5'- gGAgccGUGGC-CGUCUaugaacgaGUCCUCGAUGg -3' miRNA: 3'- gCUa--CAUCGuGCGGAg-------CGGGAGCUAC- -5' |
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31725 | 3' | -55.9 | NC_006938.1 | + | 45859 | 0.66 | 0.724085 |
Target: 5'- gCGAUG-AGCACGUCgcgauaCGCCUugacaagcggcaugUCGAUGc -3' miRNA: 3'- -GCUACaUCGUGCGGa-----GCGGG--------------AGCUAC- -5' |
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31725 | 3' | -55.9 | NC_006938.1 | + | 62670 | 0.67 | 0.684819 |
Target: 5'- aGGUGUcagGGCcaucACGaCCcCGCCCUCGGUu -3' miRNA: 3'- gCUACA---UCG----UGC-GGaGCGGGAGCUAc -5' |
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31725 | 3' | -55.9 | NC_006938.1 | + | 2937 | 0.67 | 0.674068 |
Target: 5'- aCGAUGgAGCuCGCC--GCCC-CGAUGg -3' miRNA: 3'- -GCUACaUCGuGCGGagCGGGaGCUAC- -5' |
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31725 | 3' | -55.9 | NC_006938.1 | + | 6757 | 0.67 | 0.641628 |
Target: 5'- uGAUGUAGaugguuC-CCUgGCCCUCGAUc -3' miRNA: 3'- gCUACAUCgu----GcGGAgCGGGAGCUAc -5' |
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31725 | 3' | -55.9 | NC_006938.1 | + | 46036 | 0.68 | 0.619942 |
Target: 5'- cCGGgagGUAGUGCuucgGCC-CGCCgUCGAUGg -3' miRNA: 3'- -GCUa--CAUCGUG----CGGaGCGGgAGCUAC- -5' |
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31725 | 3' | -55.9 | NC_006938.1 | + | 813 | 0.68 | 0.60911 |
Target: 5'- cCGGUGUGGC-CGCCUCcguGCCCg----- -3' miRNA: 3'- -GCUACAUCGuGCGGAG---CGGGagcuac -5' |
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31725 | 3' | -55.9 | NC_006938.1 | + | 59849 | 0.69 | 0.566061 |
Target: 5'- gGAUGcAGgAgGCCUCGUCCagGAUGa -3' miRNA: 3'- gCUACaUCgUgCGGAGCGGGagCUAC- -5' |
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31725 | 3' | -55.9 | NC_006938.1 | + | 36399 | 0.69 | 0.544816 |
Target: 5'- uCGAcGUGGCGCGCCaUCGUCU--GAUGg -3' miRNA: 3'- -GCUaCAUCGUGCGG-AGCGGGagCUAC- -5' |
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31725 | 3' | -55.9 | NC_006938.1 | + | 7663 | 0.69 | 0.544816 |
Target: 5'- aCGGUGUccgGGCACcuggccggaGcCCUCGCucgCCUCGAUGa -3' miRNA: 3'- -GCUACA---UCGUG---------C-GGAGCG---GGAGCUAC- -5' |
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31725 | 3' | -55.9 | NC_006938.1 | + | 44505 | 0.69 | 0.523836 |
Target: 5'- ---cGUAGCcCGCCUCGUCCgccgCGAc- -3' miRNA: 3'- gcuaCAUCGuGCGGAGCGGGa---GCUac -5' |
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31725 | 3' | -55.9 | NC_006938.1 | + | 2043 | 0.76 | 0.22065 |
Target: 5'- gCGGUGUAGCGCGCgaUuCCCUUGGUGa -3' miRNA: 3'- -GCUACAUCGUGCGgaGcGGGAGCUAC- -5' |
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31725 | 3' | -55.9 | NC_006938.1 | + | 53150 | 1.08 | 0.001311 |
Target: 5'- uCGAUGUAGCACGCCUCGCCCUCGAUGu -3' miRNA: 3'- -GCUACAUCGUGCGGAGCGGGAGCUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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