Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31726 | 5' | -53.2 | NC_006938.1 | + | 57046 | 0.66 | 0.863479 |
Target: 5'- uUCGAGGAGCUgaaggagaucacagCGCGCucggcauugccGUCUUcGUCGAg -3' miRNA: 3'- gAGCUUCUUGA--------------GCGCG-----------CAGAA-CGGCU- -5' |
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31726 | 5' | -53.2 | NC_006938.1 | + | 55302 | 0.66 | 0.858558 |
Target: 5'- cCUCGAagcccuugaacuggcGGAuccucuuauucgccGCUCGaCGCGUCUUGuuGc -3' miRNA: 3'- -GAGCU---------------UCU--------------UGAGC-GCGCAGAACggCu -5' |
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31726 | 5' | -53.2 | NC_006938.1 | + | 27002 | 0.66 | 0.849318 |
Target: 5'- aUCGAGGccuGC-CGCGCGgaacGCCGAc -3' miRNA: 3'- gAGCUUCu--UGaGCGCGCagaaCGGCU- -5' |
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31726 | 5' | -53.2 | NC_006938.1 | + | 2131 | 0.67 | 0.831819 |
Target: 5'- aCUCGAu--GCUC-CGUGUCgaggGCCGGu -3' miRNA: 3'- -GAGCUucuUGAGcGCGCAGaa--CGGCU- -5' |
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31726 | 5' | -53.2 | NC_006938.1 | + | 43883 | 0.67 | 0.822751 |
Target: 5'- cCUCGGAGGACagacCGUGUCggUGUCGAu -3' miRNA: 3'- -GAGCUUCUUGagc-GCGCAGa-ACGGCU- -5' |
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31726 | 5' | -53.2 | NC_006938.1 | + | 42721 | 0.68 | 0.784577 |
Target: 5'- uCUCGGGccacCUCGCGCuguGUCUUGCCc- -3' miRNA: 3'- -GAGCUUcuu-GAGCGCG---CAGAACGGcu -5' |
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31726 | 5' | -53.2 | NC_006938.1 | + | 18564 | 0.68 | 0.774611 |
Target: 5'- -cCGAGGAuGCUCGUGCa-CUUcGCCGAg -3' miRNA: 3'- gaGCUUCU-UGAGCGCGcaGAA-CGGCU- -5' |
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31726 | 5' | -53.2 | NC_006938.1 | + | 1682 | 0.68 | 0.754249 |
Target: 5'- --gGAGGAACUCGC-CGUCggucccGCUGAc -3' miRNA: 3'- gagCUUCUUGAGCGcGCAGaa----CGGCU- -5' |
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31726 | 5' | -53.2 | NC_006938.1 | + | 57122 | 0.68 | 0.743875 |
Target: 5'- uCUCGAuGAGCUCGUGC---UUGCCa- -3' miRNA: 3'- -GAGCUuCUUGAGCGCGcagAACGGcu -5' |
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31726 | 5' | -53.2 | NC_006938.1 | + | 8186 | 0.7 | 0.6248 |
Target: 5'- -aUGGAGGACaUCGCaCGUCgaGCCGAc -3' miRNA: 3'- gaGCUUCUUG-AGCGcGCAGaaCGGCU- -5' |
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31726 | 5' | -53.2 | NC_006938.1 | + | 56134 | 0.71 | 0.559257 |
Target: 5'- cCUCGAagucugagauguGGAACUCGUGCuuGUCauaGCCGAg -3' miRNA: 3'- -GAGCU------------UCUUGAGCGCG--CAGaa-CGGCU- -5' |
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31726 | 5' | -53.2 | NC_006938.1 | + | 52738 | 1.09 | 0.002132 |
Target: 5'- uCUCGAAGAACUCGCGCGUCUUGCCGAu -3' miRNA: 3'- -GAGCUUCUUGAGCGCGCAGAACGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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