Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31727 | 3' | -54 | NC_006938.1 | + | 51148 | 1.09 | 0.001925 |
Target: 5'- gCUCGCGAACGUUCUCCCGGAACCAGAc -3' miRNA: 3'- -GAGCGCUUGCAAGAGGGCCUUGGUCU- -5' |
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31727 | 3' | -54 | NC_006938.1 | + | 25688 | 0.74 | 0.386577 |
Target: 5'- -gUGUGGGCGcUUCUCCUGGAucuccuggaGCCAGAg -3' miRNA: 3'- gaGCGCUUGC-AAGAGGGCCU---------UGGUCU- -5' |
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31727 | 3' | -54 | NC_006938.1 | + | 44188 | 0.74 | 0.395519 |
Target: 5'- cCUCGCGAACcUUCUUCUGGAAgaGGAa -3' miRNA: 3'- -GAGCGCUUGcAAGAGGGCCUUggUCU- -5' |
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31727 | 3' | -54 | NC_006938.1 | + | 41277 | 0.7 | 0.630096 |
Target: 5'- gCUCGUGAGCG--CUCCCGGcgacGGCCuGGc -3' miRNA: 3'- -GAGCGCUUGCaaGAGGGCC----UUGGuCU- -5' |
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31727 | 3' | -54 | NC_006938.1 | + | 3302 | 0.69 | 0.662732 |
Target: 5'- cCUgGCGAGCcaUCUUCUGGGAgCAGGg -3' miRNA: 3'- -GAgCGCUUGcaAGAGGGCCUUgGUCU- -5' |
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31727 | 3' | -54 | NC_006938.1 | + | 39304 | 0.68 | 0.726961 |
Target: 5'- aUCGCGAc----UUCCUGGAACCGGGu -3' miRNA: 3'- gAGCGCUugcaaGAGGGCCUUGGUCU- -5' |
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31727 | 3' | -54 | NC_006938.1 | + | 58140 | 0.68 | 0.737406 |
Target: 5'- gUCgGCGAGCugUCUUCUGuGAGCCAGGa -3' miRNA: 3'- gAG-CGCUUGcaAGAGGGC-CUUGGUCU- -5' |
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31727 | 3' | -54 | NC_006938.1 | + | 51862 | 0.67 | 0.797509 |
Target: 5'- gCUCGCG---GUUCUCCgGGcuguuCCAGAa -3' miRNA: 3'- -GAGCGCuugCAAGAGGgCCuu---GGUCU- -5' |
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31727 | 3' | -54 | NC_006938.1 | + | 29254 | 0.67 | 0.786878 |
Target: 5'- -aCGUGAACugGUUgUCCCGGAACacucccaCGGAg -3' miRNA: 3'- gaGCGCUUG--CAAgAGGGCCUUG-------GUCU- -5' |
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31727 | 3' | -54 | NC_006938.1 | + | 34406 | 0.67 | 0.806996 |
Target: 5'- aCUCGUGcGACa-UCgUgCCGGAGCCAGAc -3' miRNA: 3'- -GAGCGC-UUGcaAG-AgGGCCUUGGUCU- -5' |
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31727 | 3' | -54 | NC_006938.1 | + | 12376 | 0.67 | 0.778037 |
Target: 5'- uUCaCGAACG-UCUUCCGaGAGCUGGAc -3' miRNA: 3'- gAGcGCUUGCaAGAGGGC-CUUGGUCU- -5' |
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31727 | 3' | -54 | NC_006938.1 | + | 31855 | 0.66 | 0.834342 |
Target: 5'- -gCGCGGuCGagggUgUCCUGGAGCCGGu -3' miRNA: 3'- gaGCGCUuGCa---AgAGGGCCUUGGUCu -5' |
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31727 | 3' | -54 | NC_006938.1 | + | 3405 | 0.66 | 0.834342 |
Target: 5'- cCUCGCGcccAACGgugUC-CaCCGGGACUGGGu -3' miRNA: 3'- -GAGCGC---UUGCa--AGaG-GGCCUUGGUCU- -5' |
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31727 | 3' | -54 | NC_006938.1 | + | 54988 | 0.66 | 0.843053 |
Target: 5'- cCUgGgaGGGCGUUC-CCUGGAggcACCAGGg -3' miRNA: 3'- -GAgCg-CUUGCAAGaGGGCCU---UGGUCU- -5' |
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31727 | 3' | -54 | NC_006938.1 | + | 28630 | 0.66 | 0.851548 |
Target: 5'- -gCGCcGACGUUCUUCgCGGccaugaucugGACCAGGu -3' miRNA: 3'- gaGCGcUUGCAAGAGG-GCC----------UUGGUCU- -5' |
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31727 | 3' | -54 | NC_006938.1 | + | 53676 | 0.66 | 0.843053 |
Target: 5'- -gCGgGAACGcgcccUUCUCCUGG-GCCAGc -3' miRNA: 3'- gaGCgCUUGC-----AAGAGGGCCuUGGUCu -5' |
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31727 | 3' | -54 | NC_006938.1 | + | 61864 | 0.66 | 0.816304 |
Target: 5'- gUCGCaGAuguACGgucgagCUCCCGGucccACCAGGc -3' miRNA: 3'- gAGCG-CU---UGCaa----GAGGGCCu---UGGUCU- -5' |
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31727 | 3' | -54 | NC_006938.1 | + | 25945 | 0.66 | 0.825423 |
Target: 5'- -aCGUcaGGAgGUUCUCCCcGGAACCu-- -3' miRNA: 3'- gaGCG--CUUgCAAGAGGG-CCUUGGucu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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