Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31727 | 5' | -53 | NC_006938.1 | + | 43554 | 0.66 | 0.866213 |
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Target: 5'- aGUCCuGCUgUgaGGAuGGCGUUCGg- -3' miRNA: 3'- aCAGGuCGAgAg-CCUuCUGCAAGCac -5' |
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| 31727 | 5' | -53 | NC_006938.1 | + | 12648 | 0.67 | 0.807919 |
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Target: 5'- gGUCCAGgcccucggccgcCUCgCGGAAGGCGUUgagcacgaccucggcCGUGa -3' miRNA: 3'- aCAGGUC------------GAGaGCCUUCUGCAA---------------GCAC- -5' |
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| 31727 | 5' | -53 | NC_006938.1 | + | 13394 | 0.69 | 0.733151 |
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Target: 5'- aGUCgAGCgauguaCGGAAGAUuGUUCGUGa -3' miRNA: 3'- aCAGgUCGaga---GCCUUCUG-CAAGCAC- -5' |
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| 31727 | 5' | -53 | NC_006938.1 | + | 43861 | 0.69 | 0.712865 |
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Target: 5'- gGUCCGGCggguccugcagaaccCUCGGAGGACaGacCGUGu -3' miRNA: 3'- aCAGGUCGa--------------GAGCCUUCUG-CaaGCAC- -5' |
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| 31727 | 5' | -53 | NC_006938.1 | + | 45381 | 0.69 | 0.711789 |
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Target: 5'- gUGUCCGGC-CgcgCGGAGGGCGUcCa-- -3' miRNA: 3'- -ACAGGUCGaGa--GCCUUCUGCAaGcac -5' |
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| 31727 | 5' | -53 | NC_006938.1 | + | 56418 | 0.7 | 0.667108 |
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Target: 5'- -cUCCAGCgggagcgCUCGGAccgcuccGGACGgagcUCGUGg -3' miRNA: 3'- acAGGUCGa------GAGCCU-------UCUGCa---AGCAC- -5' |
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| 31727 | 5' | -53 | NC_006938.1 | + | 51076 | 0.7 | 0.639542 |
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Target: 5'- -cUCCGGuCUCUauccgauugaaccagCGGAAGGCGaUCGUGg -3' miRNA: 3'- acAGGUC-GAGA---------------GCCUUCUGCaAGCAC- -5' |
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| 31727 | 5' | -53 | NC_006938.1 | + | 12411 | 0.71 | 0.569133 |
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Target: 5'- cGUCUGGUUC-CGGGAGaACGUUCGc- -3' miRNA: 3'- aCAGGUCGAGaGCCUUC-UGCAAGCac -5' |
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| 31727 | 5' | -53 | NC_006938.1 | + | 51183 | 1.09 | 0.002011 |
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Target: 5'- uUGUCCAGCUCUCGGAAGACGUUCGUGa -3' miRNA: 3'- -ACAGGUCGAGAGCCUUCUGCAAGCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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