Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31731 | 3' | -59.1 | NC_006938.1 | + | 36105 | 0.66 | 0.563103 |
Target: 5'- -uCUcCUCGugcuCCUCGGCCGCUCc -3' miRNA: 3'- ucGAcGAGCuacuGGAGCCGGCGAGc -5' |
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31731 | 3' | -59.1 | NC_006938.1 | + | 23138 | 0.66 | 0.531953 |
Target: 5'- cGGCgcgGCgUCGGUGAUCU-GGCCucGCUUGa -3' miRNA: 3'- -UCGa--CG-AGCUACUGGAgCCGG--CGAGC- -5' |
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31731 | 3' | -59.1 | NC_006938.1 | + | 29687 | 0.66 | 0.531953 |
Target: 5'- aGGgaGCUCGAUGACCggaGGCUugugguGCaUCa -3' miRNA: 3'- -UCgaCGAGCUACUGGag-CCGG------CG-AGc -5' |
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31731 | 3' | -59.1 | NC_006938.1 | + | 9963 | 0.67 | 0.501446 |
Target: 5'- gAGgaGCUCcagGAggaCUCGGCCGCUaCGg -3' miRNA: 3'- -UCgaCGAGcuaCUg--GAGCCGGCGA-GC- -5' |
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31731 | 3' | -59.1 | NC_006938.1 | + | 39530 | 0.67 | 0.501446 |
Target: 5'- aGGUUGCcaaguUCGGUGGCa-CGGCCaccGCUCGc -3' miRNA: 3'- -UCGACG-----AGCUACUGgaGCCGG---CGAGC- -5' |
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31731 | 3' | -59.1 | NC_006938.1 | + | 25576 | 0.67 | 0.501446 |
Target: 5'- -cCUGCUCcGUGAcguuccaacCCUCGGCCGUgaaCGg -3' miRNA: 3'- ucGACGAGcUACU---------GGAGCCGGCGa--GC- -5' |
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31731 | 3' | -59.1 | NC_006938.1 | + | 10601 | 0.67 | 0.501446 |
Target: 5'- aAGCUGgUCGAUGcaccacCCUCGGgaGcCUCGa -3' miRNA: 3'- -UCGACgAGCUACu-----GGAGCCggC-GAGC- -5' |
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31731 | 3' | -59.1 | NC_006938.1 | + | 30858 | 0.67 | 0.491446 |
Target: 5'- gAGCUGCUUGcgGA--UCGGCuCGC-CGg -3' miRNA: 3'- -UCGACGAGCuaCUggAGCCG-GCGaGC- -5' |
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31731 | 3' | -59.1 | NC_006938.1 | + | 11241 | 0.67 | 0.491446 |
Target: 5'- aAGUUGUugUCGAUGccGCCcUGGCCGC-CGa -3' miRNA: 3'- -UCGACG--AGCUAC--UGGaGCCGGCGaGC- -5' |
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31731 | 3' | -59.1 | NC_006938.1 | + | 44664 | 0.67 | 0.471732 |
Target: 5'- cGC-GCUCGGUGGCC-CGGUC-CUUGc -3' miRNA: 3'- uCGaCGAGCUACUGGaGCCGGcGAGC- -5' |
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31731 | 3' | -59.1 | NC_006938.1 | + | 30727 | 0.67 | 0.462026 |
Target: 5'- cAGCUGCccUCGA--GCCacgCGGCCaGCUUGg -3' miRNA: 3'- -UCGACG--AGCUacUGGa--GCCGG-CGAGC- -5' |
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31731 | 3' | -59.1 | NC_006938.1 | + | 28878 | 0.67 | 0.462026 |
Target: 5'- cAGCUGUcCGcccuUCUgGGCCGCUCGg -3' miRNA: 3'- -UCGACGaGCuacuGGAgCCGGCGAGC- -5' |
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31731 | 3' | -59.1 | NC_006938.1 | + | 49606 | 0.68 | 0.452426 |
Target: 5'- ---aGCUUGcUGAgCUCGGCCccGCUCGg -3' miRNA: 3'- ucgaCGAGCuACUgGAGCCGG--CGAGC- -5' |
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31731 | 3' | -59.1 | NC_006938.1 | + | 7985 | 0.68 | 0.452426 |
Target: 5'- --gUGCUCGgcGACC-CGGCCcaGCUCc -3' miRNA: 3'- ucgACGAGCuaCUGGaGCCGG--CGAGc -5' |
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31731 | 3' | -59.1 | NC_006938.1 | + | 12636 | 0.68 | 0.433559 |
Target: 5'- uGGCgaaGuCUCGGUccaGGcCCUCGGCCGcCUCGc -3' miRNA: 3'- -UCGa--C-GAGCUA---CU-GGAGCCGGC-GAGC- -5' |
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31731 | 3' | -59.1 | NC_006938.1 | + | 27583 | 0.68 | 0.406138 |
Target: 5'- uGGCggUGCgagUGAUGGCCUCGG-CGUUCc -3' miRNA: 3'- -UCG--ACGa--GCUACUGGAGCCgGCGAGc -5' |
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31731 | 3' | -59.1 | NC_006938.1 | + | 42490 | 0.69 | 0.397243 |
Target: 5'- aAGgUGgUCGucGUGAUC-CGGCCGUUCGg -3' miRNA: 3'- -UCgACgAGC--UACUGGaGCCGGCGAGC- -5' |
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31731 | 3' | -59.1 | NC_006938.1 | + | 29205 | 0.69 | 0.371321 |
Target: 5'- uGCaGCgUGGUG-CCUCGGUgGCUCGg -3' miRNA: 3'- uCGaCGaGCUACuGGAGCCGgCGAGC- -5' |
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31731 | 3' | -59.1 | NC_006938.1 | + | 21628 | 0.69 | 0.371321 |
Target: 5'- cGCUGCUggacggCGGUGACCagggcgacgUgGGCCGCgcgCGg -3' miRNA: 3'- uCGACGA------GCUACUGG---------AgCCGGCGa--GC- -5' |
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31731 | 3' | -59.1 | NC_006938.1 | + | 16571 | 0.7 | 0.308018 |
Target: 5'- gAGCUGCUCaaaGAgccGAUCUCGGagGCUCGu -3' miRNA: 3'- -UCGACGAG---CUa--CUGGAGCCggCGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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