Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31734 | 3' | -53.9 | NC_006938.1 | + | 11444 | 0.66 | 0.859817 |
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Target: 5'- -cUGGGUCAgGUCGGcCAgcaGGGAGc -3' miRNA: 3'- gcACCCAGUgCAGCCuGUaggUCUUC- -5' |
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| 31734 | 3' | -53.9 | NC_006938.1 | + | 25665 | 0.66 | 0.834342 |
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Target: 5'- gCGUGGGUCACGccgagCGaGACA-CgGGAc- -3' miRNA: 3'- -GCACCCAGUGCa----GC-CUGUaGgUCUuc -5' |
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| 31734 | 3' | -53.9 | NC_006938.1 | + | 24595 | 0.66 | 0.825423 |
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Target: 5'- aCGUcGGGaucuggaccUCGCcaCGGGCAUCCGGGAu -3' miRNA: 3'- -GCA-CCC---------AGUGcaGCCUGUAGGUCUUc -5' |
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| 31734 | 3' | -53.9 | NC_006938.1 | + | 46030 | 0.66 | 0.812603 |
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Target: 5'- gCGUGGGccgagauugacagCACGaCGGACAcCCAGGu- -3' miRNA: 3'- -GCACCCa------------GUGCaGCCUGUaGGUCUuc -5' |
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| 31734 | 3' | -53.9 | NC_006938.1 | + | 23252 | 0.67 | 0.806996 |
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Target: 5'- aGU-GGUCgGCGaccgaggCGGACGUCCAGGAc -3' miRNA: 3'- gCAcCCAG-UGCa------GCCUGUAGGUCUUc -5' |
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| 31734 | 3' | -53.9 | NC_006938.1 | + | 5372 | 0.67 | 0.798465 |
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Target: 5'- uCGUGGGagacccgacgagcgcCACGUUGG-CGUCCGcGAGGa -3' miRNA: 3'- -GCACCCa--------------GUGCAGCCuGUAGGU-CUUC- -5' |
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| 31734 | 3' | -53.9 | NC_006938.1 | + | 52685 | 0.74 | 0.413797 |
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Target: 5'- cCGUGGGgagugggACGUCGGcuaugGCAUCCAGGAc -3' miRNA: 3'- -GCACCCag-----UGCAGCC-----UGUAGGUCUUc -5' |
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| 31734 | 3' | -53.9 | NC_006938.1 | + | 43967 | 1.08 | 0.002231 |
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Target: 5'- uCGUGGGUCACGUCGGACAUCCAGAAGc -3' miRNA: 3'- -GCACCCAGUGCAGCCUGUAGGUCUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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