Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31738 | 3' | -57.2 | NC_006938.1 | + | 58572 | 0.66 | 0.67924 |
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Target: 5'- aUCGGUGGggcGCUCGUggaUGG-CCUGg -3' miRNA: 3'- gGGCCAUCa--CGAGCGag-ACCaGGACg -5' |
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| 31738 | 3' | -57.2 | NC_006938.1 | + | 16190 | 0.66 | 0.657966 |
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Target: 5'- uCCUGGaacaUGCucaaccUCGCgCUGGUCCUGUu -3' miRNA: 3'- -GGGCCauc-ACG------AGCGaGACCAGGACG- -5' |
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| 31738 | 3' | -57.2 | NC_006938.1 | + | 28025 | 0.66 | 0.636602 |
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Target: 5'- -gCGaGUGGUGCauggaGCUCUGGUUCgaUGCc -3' miRNA: 3'- ggGC-CAUCACGag---CGAGACCAGG--ACG- -5' |
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| 31738 | 3' | -57.2 | NC_006938.1 | + | 6606 | 0.66 | 0.636602 |
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Target: 5'- -aCGGgcGUGCUCaGCUCUGuG-CCauUGCu -3' miRNA: 3'- ggGCCauCACGAG-CGAGAC-CaGG--ACG- -5' |
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| 31738 | 3' | -57.2 | NC_006938.1 | + | 43230 | 0.67 | 0.615222 |
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Target: 5'- aCCCGGccAGUGCcuUCGCggCgaacagcacggUGGcCCUGCa -3' miRNA: 3'- -GGGCCa-UCACG--AGCGa-G-----------ACCaGGACG- -5' |
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| 31738 | 3' | -57.2 | NC_006938.1 | + | 23348 | 0.69 | 0.510407 |
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Target: 5'- cCCCGucacgcauGUGCUCcacgauGCUCUGGUCCgugaccugUGCa -3' miRNA: 3'- -GGGCcau-----CACGAG------CGAGACCAGG--------ACG- -5' |
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| 31738 | 3' | -57.2 | NC_006938.1 | + | 50390 | 0.71 | 0.369853 |
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Target: 5'- uCCCGGUGGUGCcgggUGCgaaCcGG-CCUGCc -3' miRNA: 3'- -GGGCCAUCACGa---GCGa--GaCCaGGACG- -5' |
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| 31738 | 3' | -57.2 | NC_006938.1 | + | 40494 | 1.14 | 0.000395 |
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Target: 5'- gCCCGGUAGUGCUCGCUCUGGUCCUGCa -3' miRNA: 3'- -GGGCCAUCACGAGCGAGACCAGGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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