Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 3174 | 3' | -54 | NC_001501.1 | + | 2311 | 0.66 | 0.263989 |
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Target: 5'- gGGC-AGCCCCugCAAGUGuu-GACCCUa -3' miRNA: 3'- -CUGcUCGGGG--GUUUACuuuCUGGGGg -5' |
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| 3174 | 3' | -54 | NC_001501.1 | + | 8208 | 0.66 | 0.256227 |
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Target: 5'- --gGGGCUcguCCgGGAUcGGGAGACCCCUg -3' miRNA: 3'- cugCUCGG---GGgUUUA-CUUUCUGGGGG- -5' |
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| 3174 | 3' | -54 | NC_001501.1 | + | 8010 | 0.66 | 0.240537 |
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Target: 5'- gGACuGGCgCCCCGAGUGAGggguuguGGGCUCUUu -3' miRNA: 3'- -CUGcUCG-GGGGUUUACUU-------UCUGGGGG- -5' |
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| 3174 | 3' | -54 | NC_001501.1 | + | 7806 | 0.67 | 0.227039 |
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Target: 5'- aGAUG-GUCCCCAGAUGcgguccaGCCCUCa -3' miRNA: 3'- -CUGCuCGGGGGUUUACuuuc---UGGGGG- -5' |
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| 3174 | 3' | -54 | NC_001501.1 | + | 133 | 0.67 | 0.200716 |
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Target: 5'- aACG-GCCCCCGAAgucccUGGGAcGuCUCCCa -3' miRNA: 3'- cUGCuCGGGGGUUU-----ACUUU-CuGGGGG- -5' |
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| 3174 | 3' | -54 | NC_001501.1 | + | 7524 | 0.68 | 0.182752 |
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Target: 5'- ---uAGUCUCCAgaaaaaggggggAAUGAAAGACCCCa -3' miRNA: 3'- cugcUCGGGGGU------------UUACUUUCUGGGGg -5' |
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| 3174 | 3' | -54 | NC_001501.1 | + | 957 | 0.71 | 0.102212 |
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Target: 5'- aGACggGAGCCCCCu-GUGGccGACUCCa -3' miRNA: 3'- -CUG--CUCGGGGGuuUACUuuCUGGGGg -5' |
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| 3174 | 3' | -54 | NC_001501.1 | + | 5094 | 0.72 | 0.095675 |
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Target: 5'- -cCGAGCCCgCAAcacgccGGGCCCCCa -3' miRNA: 3'- cuGCUCGGGgGUUuacuu-UCUGGGGG- -5' |
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| 3174 | 3' | -54 | NC_001501.1 | + | 8226 | 1.12 | 4.6e-05 |
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Target: 5'- gGACGAGCCCCCAAAUGAAAGACCCCCg -3' miRNA: 3'- -CUGCUCGGGGGUUUACUUUCUGGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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