Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31741 | 3' | -55.9 | NC_006938.1 | + | 53688 | 0.66 | 0.725166 |
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Target: 5'- cCUUCUc-CUGGGCCagcucgacGGAGGCCUgauaGGa -3' miRNA: 3'- -GAAGAacGACCCGG--------CCUUCGGGaa--CC- -5' |
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| 31741 | 3' | -55.9 | NC_006938.1 | + | 43440 | 0.66 | 0.725166 |
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Target: 5'- -----cGCUGGuuGUCGGAGGCCCa-GGa -3' miRNA: 3'- gaagaaCGACC--CGGCCUUCGGGaaCC- -5' |
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| 31741 | 3' | -55.9 | NC_006938.1 | + | 11573 | 0.66 | 0.714606 |
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Target: 5'- -gUCggGCggcaGGuGgCGGAGGCCUUUGGc -3' miRNA: 3'- gaAGaaCGa---CC-CgGCCUUCGGGAACC- -5' |
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| 31741 | 3' | -55.9 | NC_006938.1 | + | 10670 | 0.67 | 0.66083 |
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Target: 5'- -aUCUUGCUGaGGauCCGGucgaacgaggucGAGCCCgaGGu -3' miRNA: 3'- gaAGAACGAC-CC--GGCC------------UUCGGGaaCC- -5' |
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| 31741 | 3' | -55.9 | NC_006938.1 | + | 5068 | 0.67 | 0.648865 |
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Target: 5'- -gUC-UGCUGGGCUGGugcguauggcaucAGGUCCcUGGc -3' miRNA: 3'- gaAGaACGACCCGGCC-------------UUCGGGaACC- -5' |
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| 31741 | 3' | -55.9 | NC_006938.1 | + | 46670 | 0.69 | 0.510402 |
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Target: 5'- gCUUCUccgcguagUGCUGGGCCuugcGGAGGUcguCCUcGGg -3' miRNA: 3'- -GAAGA--------ACGACCCGG----CCUUCG---GGAaCC- -5' |
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| 31741 | 3' | -55.9 | NC_006938.1 | + | 16283 | 0.7 | 0.479746 |
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Target: 5'- -----cGCUGGGUCGGcgacAGCCCcUGGc -3' miRNA: 3'- gaagaaCGACCCGGCCu---UCGGGaACC- -5' |
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| 31741 | 3' | -55.9 | NC_006938.1 | + | 13307 | 0.7 | 0.458831 |
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Target: 5'- -aUCUUGCUGcGCCGGAcccgccggaccccGGCCUggaUGGc -3' miRNA: 3'- gaAGAACGACcCGGCCU-------------UCGGGa--ACC- -5' |
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| 31741 | 3' | -55.9 | NC_006938.1 | + | 38808 | 1.11 | 0.000676 |
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Target: 5'- cCUUCUUGCUGGGCCGGAAGCCCUUGGg -3' miRNA: 3'- -GAAGAACGACCCGGCCUUCGGGAACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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