Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31743 | 5' | -52.4 | NC_006938.1 | + | 28655 | 0.66 | 0.890854 |
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Target: 5'- cACGUGGC-UGGagCUCCgUCGCgGCugGg -3' miRNA: 3'- -UGUACUGcAUCaaGAGG-AGCG-CGugC- -5' |
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| 31743 | 5' | -52.4 | NC_006938.1 | + | 36076 | 0.66 | 0.875753 |
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Target: 5'- -gGUGACGgagaccgGGUauccgcucuccaUCUCCUCGUGCuccuCGg -3' miRNA: 3'- ugUACUGCa------UCA------------AGAGGAGCGCGu---GC- -5' |
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| 31743 | 5' | -52.4 | NC_006938.1 | + | 27904 | 0.66 | 0.875753 |
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Target: 5'- ---cGACGgacAGcaUCUCCUCGCGcCACa -3' miRNA: 3'- uguaCUGCa--UCa-AGAGGAGCGC-GUGc -5' |
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| 31743 | 5' | -52.4 | NC_006938.1 | + | 24949 | 0.66 | 0.867817 |
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Target: 5'- cACGUGAUGUuaggCUCCUguuaaccaaCGCGUGCGa -3' miRNA: 3'- -UGUACUGCAucaaGAGGA---------GCGCGUGC- -5' |
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| 31743 | 5' | -52.4 | NC_006938.1 | + | 27505 | 0.67 | 0.823643 |
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Target: 5'- ---cGACGUAGaUCUCCUCcucgaagagggugGCGUugGc -3' miRNA: 3'- uguaCUGCAUCaAGAGGAG-------------CGCGugC- -5' |
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| 31743 | 5' | -52.4 | NC_006938.1 | + | 37823 | 1.08 | 0.002666 |
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Target: 5'- cACAUGACGUAGUUCUCCUCGCGCACGu -3' miRNA: 3'- -UGUACUGCAUCAAGAGGAGCGCGUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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