Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31744 | 5' | -53.2 | NC_006938.1 | + | 410 | 0.66 | 0.83347 |
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Target: 5'- uGGCuUGGcagccGUCGCCAUCUUGG-GUAUc -3' miRNA: 3'- cCCG-ACC-----UAGCGGUAGAGCUaCAUGa -5' |
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| 31744 | 5' | -53.2 | NC_006938.1 | + | 12024 | 0.67 | 0.786221 |
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Target: 5'- uGGCgGcGGUUGCgAUCUCGAUGaACg -3' miRNA: 3'- cCCGaC-CUAGCGgUAGAGCUACaUGa -5' |
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| 31744 | 5' | -53.2 | NC_006938.1 | + | 15114 | 0.71 | 0.582027 |
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Target: 5'- uGGGCcGGAccucCGCCAUCUUGAUGc--- -3' miRNA: 3'- -CCCGaCCUa---GCGGUAGAGCUACauga -5' |
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| 31744 | 5' | -53.2 | NC_006938.1 | + | 16770 | 0.75 | 0.354316 |
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Target: 5'- cGGGCUgcaGGAucUCGaCCAUCUCGAUGcGCc -3' miRNA: 3'- -CCCGA---CCU--AGC-GGUAGAGCUACaUGa -5' |
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| 31744 | 5' | -53.2 | NC_006938.1 | + | 55470 | 0.76 | 0.337651 |
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Target: 5'- -cGC-GGGUCGCCAUCUCGAUGa--- -3' miRNA: 3'- ccCGaCCUAGCGGUAGAGCUACauga -5' |
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| 31744 | 5' | -53.2 | NC_006938.1 | + | 37286 | 1.1 | 0.001696 |
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Target: 5'- cGGGCUGGAUCGCCAUCUCGAUGUACUu -3' miRNA: 3'- -CCCGACCUAGCGGUAGAGCUACAUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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