Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31745 | 5' | -56.7 | NC_006938.1 | + | 57787 | 0.66 | 0.61262 |
Target: 5'- uCCgAGAGCUCCGU-CACguuCUCGUCGAc -3' miRNA: 3'- -GG-UCUCGAGGUAcGUGgu-GAGCGGUU- -5' |
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31745 | 5' | -56.7 | NC_006938.1 | + | 60709 | 0.66 | 0.64549 |
Target: 5'- cUCGGAGCguuaUgAUGguCCGCUUGCCGu -3' miRNA: 3'- -GGUCUCGa---GgUACguGGUGAGCGGUu -5' |
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31745 | 5' | -56.7 | NC_006938.1 | + | 61350 | 0.67 | 0.601684 |
Target: 5'- gCCGGAGCacgUCUAUGaUGCCGCccUCGCCc- -3' miRNA: 3'- -GGUCUCG---AGGUAC-GUGGUG--AGCGGuu -5' |
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31745 | 5' | -56.7 | NC_006938.1 | + | 61812 | 0.66 | 0.656436 |
Target: 5'- aCCAGAGCaggCCugGgGCCAgUUGCCAc -3' miRNA: 3'- -GGUCUCGa--GGuaCgUGGUgAGCGGUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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