Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31747 | 3' | -57.3 | NC_006938.1 | + | 47168 | 0.66 | 0.635288 |
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Target: 5'- uCGAGcUCgaUCGGCUCGAGGU-UCGGCg- -3' miRNA: 3'- -GCUC-AG--GGUCGAGCUCCAuGGUCGac -5' |
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| 31747 | 3' | -57.3 | NC_006938.1 | + | 53944 | 0.67 | 0.570862 |
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Target: 5'- -aGGUCCCAG-UCGaAGGU-CCAGCg- -3' miRNA: 3'- gcUCAGGGUCgAGC-UCCAuGGUCGac -5' |
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| 31747 | 3' | -57.3 | NC_006938.1 | + | 37506 | 0.67 | 0.560245 |
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Target: 5'- aGA-UCCCcgAGCUCGAGGccGCCGccGCUGg -3' miRNA: 3'- gCUcAGGG--UCGAGCUCCa-UGGU--CGAC- -5' |
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| 31747 | 3' | -57.3 | NC_006938.1 | + | 39980 | 0.68 | 0.528756 |
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Target: 5'- cCGAGcUCgaagCCAGCcgCGAGG-ACCAGCUu -3' miRNA: 3'- -GCUC-AG----GGUCGa-GCUCCaUGGUCGAc -5' |
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| 31747 | 3' | -57.3 | NC_006938.1 | + | 43307 | 0.68 | 0.50813 |
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Target: 5'- uCGGGUCCCAGC-CGAacaGG-GCUGGCUu -3' miRNA: 3'- -GCUCAGGGUCGaGCU---CCaUGGUCGAc -5' |
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| 31747 | 3' | -57.3 | NC_006938.1 | + | 12520 | 0.68 | 0.487851 |
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Target: 5'- gCGGGacagcCCCGGCcCGAGGUuCCGGCa- -3' miRNA: 3'- -GCUCa----GGGUCGaGCUCCAuGGUCGac -5' |
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| 31747 | 3' | -57.3 | NC_006938.1 | + | 32478 | 0.69 | 0.477855 |
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Target: 5'- gGAGUUCCAGUUCaucGGGUgggACgAGCUGa -3' miRNA: 3'- gCUCAGGGUCGAGc--UCCA---UGgUCGAC- -5' |
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| 31747 | 3' | -57.3 | NC_006938.1 | + | 30718 | 0.69 | 0.429489 |
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Target: 5'- aCGAuGUCCCAGCugcccUCGAGccacgcgGCCAGCUu -3' miRNA: 3'- -GCU-CAGGGUCG-----AGCUCca-----UGGUCGAc -5' |
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| 31747 | 3' | -57.3 | NC_006938.1 | + | 42109 | 0.7 | 0.401889 |
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Target: 5'- -uGGUCCCcGC-CGAGGUccgucuCCAGCUGu -3' miRNA: 3'- gcUCAGGGuCGaGCUCCAu-----GGUCGAC- -5' |
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| 31747 | 3' | -57.3 | NC_006938.1 | + | 33408 | 1.09 | 0.000723 |
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Target: 5'- cCGAGUCCCAGCUCGAGGUACCAGCUGg -3' miRNA: 3'- -GCUCAGGGUCGAGCUCCAUGGUCGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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