Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31750 | 5' | -55.9 | NC_006938.1 | + | 32933 | 0.66 | 0.705445 |
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Target: 5'- -cGCGGCUCGGGCg---CGUUcGUGCu -3' miRNA: 3'- guCGUCGAGCUCGgucaGCAA-CACGc -5' |
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| 31750 | 5' | -55.9 | NC_006938.1 | + | 6308 | 0.66 | 0.694708 |
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Target: 5'- gAGCGGCUCGAggGCCuGUCGauagGUcaucugGCGa -3' miRNA: 3'- gUCGUCGAGCU--CGGuCAGCaa--CA------CGC- -5' |
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| 31750 | 5' | -55.9 | NC_006938.1 | + | 45463 | 0.66 | 0.68391 |
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Target: 5'- aAGCAGCaCGGGUuggCAGUCGcggGUGCc -3' miRNA: 3'- gUCGUCGaGCUCG---GUCAGCaa-CACGc -5' |
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| 31750 | 5' | -55.9 | NC_006938.1 | + | 59159 | 0.67 | 0.607545 |
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Target: 5'- gGGCAGCggUGAGCaGGUCGaUGUcGCGc -3' miRNA: 3'- gUCGUCGa-GCUCGgUCAGCaACA-CGC- -5' |
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| 31750 | 5' | -55.9 | NC_006938.1 | + | 9764 | 0.69 | 0.49055 |
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Target: 5'- cCAGUGGCUCcaacgGAGCUucGGUCGUUG-GCa -3' miRNA: 3'- -GUCGUCGAG-----CUCGG--UCAGCAACaCGc -5' |
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| 31750 | 5' | -55.9 | NC_006938.1 | + | 63359 | 0.74 | 0.274377 |
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Target: 5'- gGGCGGCggCGAGCCGGUCGcccacGCGg -3' miRNA: 3'- gUCGUCGa-GCUCGGUCAGCaaca-CGC- -5' |
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| 31750 | 5' | -55.9 | NC_006938.1 | + | 31752 | 1.08 | 0.001163 |
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Target: 5'- cCAGCAGCUCGAGCCAGUCGUUGUGCGc -3' miRNA: 3'- -GUCGUCGAGCUCGGUCAGCAACACGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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