Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31752 | 3' | -59.8 | NC_006938.1 | + | 26948 | 0.66 | 0.504035 |
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Target: 5'- uGGGccUGUCCCUCCGGAGUGuGGCa- -3' miRNA: 3'- cUCCacAUGGGGAGGUCUCAC-CCGgu -5' |
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| 31752 | 3' | -59.8 | NC_006938.1 | + | 32852 | 0.66 | 0.484026 |
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Target: 5'- cAGGUGUGCCuCCUCCGGuGUcuGGaaguCCAc -3' miRNA: 3'- cUCCACAUGG-GGAGGUCuCA--CCc---GGU- -5' |
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| 31752 | 3' | -59.8 | NC_006938.1 | + | 41338 | 0.66 | 0.474165 |
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Target: 5'- -uGGcgGUGCCUCgCCGGAG-GGGCUg -3' miRNA: 3'- cuCCa-CAUGGGGaGGUCUCaCCCGGu -5' |
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| 31752 | 3' | -59.8 | NC_006938.1 | + | 20874 | 0.68 | 0.381733 |
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Target: 5'- -uGGUGUcgACCCCUCCaccugaggaGGuGGUGGaGCCGc -3' miRNA: 3'- cuCCACA--UGGGGAGG---------UC-UCACC-CGGU- -5' |
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| 31752 | 3' | -59.8 | NC_006938.1 | + | 35339 | 0.69 | 0.324582 |
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Target: 5'- uAGGUGUGCCCCaUCCAGAuccGcGCCGc -3' miRNA: 3'- cUCCACAUGGGG-AGGUCUca-CcCGGU- -5' |
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| 31752 | 3' | -59.8 | NC_006938.1 | + | 10352 | 0.7 | 0.274045 |
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Target: 5'- cGAGGguUGUGaccucUCCCUCCAG-GUGGGUCu -3' miRNA: 3'- -CUCC--ACAU-----GGGGAGGUCuCACCCGGu -5' |
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| 31752 | 3' | -59.8 | NC_006938.1 | + | 21396 | 0.71 | 0.241947 |
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Target: 5'- cGGGUGacaaGCCCCUCggaGGAggcGUGGGCCGa -3' miRNA: 3'- cUCCACa---UGGGGAGg--UCU---CACCCGGU- -5' |
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| 31752 | 3' | -59.8 | NC_006938.1 | + | 31046 | 1.08 | 0.00048 |
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Target: 5'- gGAGGUGUACCCCUCCAGAGUGGGCCAg -3' miRNA: 3'- -CUCCACAUGGGGAGGUCUCACCCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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