Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31752 | 5' | -53.7 | NC_006938.1 | + | 60145 | 0.66 | 0.836175 |
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Target: 5'- cCUCGaCCCAguCCCGGUGGACaccguUGGGcGCg -3' miRNA: 3'- -GAGCaGGGU--GGGCUACUUG-----ACCU-UGa -5' |
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| 31752 | 5' | -53.7 | NC_006938.1 | + | 39486 | 0.66 | 0.827227 |
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Target: 5'- aCUUGUCCCuCCCGAUGAAUc------ -3' miRNA: 3'- -GAGCAGGGuGGGCUACUUGaccuuga -5' |
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| 31752 | 5' | -53.7 | NC_006938.1 | + | 4787 | 0.66 | 0.818075 |
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Target: 5'- cCUCGgccaaCCGCCuCGcUGAuCUGGAACa -3' miRNA: 3'- -GAGCag---GGUGG-GCuACUuGACCUUGa -5' |
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| 31752 | 5' | -53.7 | NC_006938.1 | + | 63375 | 0.67 | 0.769609 |
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Target: 5'- gUCG-CCCACgCGGUGGAcCUGGGuGCa -3' miRNA: 3'- gAGCaGGGUGgGCUACUU-GACCU-UGa -5' |
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| 31752 | 5' | -53.7 | NC_006938.1 | + | 10709 | 0.67 | 0.759449 |
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Target: 5'- -gUGUaCCGCuuGAaGAGCUGGAACg -3' miRNA: 3'- gaGCAgGGUGggCUaCUUGACCUUGa -5' |
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| 31752 | 5' | -53.7 | NC_006938.1 | + | 5605 | 0.7 | 0.630648 |
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Target: 5'- -cCGUCCCACCCGuc---CUGGAAg- -3' miRNA: 3'- gaGCAGGGUGGGCuacuuGACCUUga -5' |
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| 31752 | 5' | -53.7 | NC_006938.1 | + | 57362 | 0.71 | 0.565145 |
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Target: 5'- cCUCGaCCaGCuCCGAguUGGACUGGAGCa -3' miRNA: 3'- -GAGCaGGgUG-GGCU--ACUUGACCUUGa -5' |
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| 31752 | 5' | -53.7 | NC_006938.1 | + | 46632 | 0.75 | 0.326293 |
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Target: 5'- uUCGUCCCugCCacagguGGUGGcCUGGGACUg -3' miRNA: 3'- gAGCAGGGugGG------CUACUuGACCUUGA- -5' |
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| 31752 | 5' | -53.7 | NC_006938.1 | + | 31081 | 1.07 | 0.0024 |
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Target: 5'- gCUCGUCCCACCCGAUGAACUGGAACUc -3' miRNA: 3'- -GAGCAGGGUGGGCUACUUGACCUUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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