Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31753 | 5' | -58.1 | NC_006938.1 | + | 52814 | 0.7 | 0.351589 |
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Target: 5'- cUCUCGGCagGCCAUUggcaCCACGGAcGGCa -3' miRNA: 3'- -AGAGCCGggUGGUAG----GGUGCCUcUUG- -5' |
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| 31753 | 5' | -58.1 | NC_006938.1 | + | 39395 | 0.71 | 0.31243 |
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Target: 5'- cUCUCGGgcucCCCGCUcucuUCUCaACGGAGGACa -3' miRNA: 3'- -AGAGCC----GGGUGGu---AGGG-UGCCUCUUG- -5' |
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| 31753 | 5' | -58.1 | NC_006938.1 | + | 22955 | 0.72 | 0.27667 |
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Target: 5'- --cCGGUCCGCCAgCUC-CGGGGAGCg -3' miRNA: 3'- agaGCCGGGUGGUaGGGuGCCUCUUG- -5' |
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| 31753 | 5' | -58.1 | NC_006938.1 | + | 54155 | 0.73 | 0.244254 |
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Target: 5'- gCUCGGCCCA--GUCCCACGuGGAccGCa -3' miRNA: 3'- aGAGCCGGGUggUAGGGUGCcUCU--UG- -5' |
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| 31753 | 5' | -58.1 | NC_006938.1 | + | 23799 | 0.73 | 0.238162 |
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Target: 5'- --cCGGCCCucuCCAcUCCCacacagaaACGGAGAACg -3' miRNA: 3'- agaGCCGGGu--GGU-AGGG--------UGCCUCUUG- -5' |
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| 31753 | 5' | -58.1 | NC_006938.1 | + | 56401 | 0.74 | 0.220646 |
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Target: 5'- gCUCGGCCauuCGCCGUCUCcaGCGG-GAGCg -3' miRNA: 3'- aGAGCCGG---GUGGUAGGG--UGCCuCUUG- -5' |
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| 31753 | 5' | -58.1 | NC_006938.1 | + | 48181 | 0.74 | 0.215057 |
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Target: 5'- gCUCGGCaaGCCcaAUCCCACGGGcGACa -3' miRNA: 3'- aGAGCCGggUGG--UAGGGUGCCUcUUG- -5' |
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| 31753 | 5' | -58.1 | NC_006938.1 | + | 29225 | 0.79 | 0.101693 |
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Target: 5'- gCUCGGCgUgACCAucucUCCCugGGAGAACg -3' miRNA: 3'- aGAGCCG-GgUGGU----AGGGugCCUCUUG- -5' |
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| 31753 | 5' | -58.1 | NC_006938.1 | + | 30577 | 1.1 | 0.000538 |
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Target: 5'- cUCUCGGCCCACCAUCCCACGGAGAACg -3' miRNA: 3'- -AGAGCCGGGUGGUAGGGUGCCUCUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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