Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31754 | 3' | -54.8 | NC_006938.1 | + | 10273 | 0.66 | 0.757848 |
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Target: 5'- cGCGccUGACaug----CCCUGGGAGGACu -3' miRNA: 3'- -CGC--ACUGguagagaGGGACCCUCUUG- -5' |
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| 31754 | 3' | -54.8 | NC_006938.1 | + | 20856 | 0.67 | 0.694708 |
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Target: 5'- -gGUGGCCcgagaccucggcGUCUCcaggCUCUGGGuGAACa -3' miRNA: 3'- cgCACUGG------------UAGAGa---GGGACCCuCUUG- -5' |
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| 31754 | 3' | -54.8 | NC_006938.1 | + | 9483 | 0.68 | 0.681745 |
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Target: 5'- uGCGaGACCAUCgccgccaccaaUCCCUGcGGcGAGCa -3' miRNA: 3'- -CGCaCUGGUAGag---------AGGGAC-CCuCUUG- -5' |
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| 31754 | 3' | -54.8 | NC_006938.1 | + | 47096 | 0.68 | 0.651267 |
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Target: 5'- uGCGUGuugcccaggaGCCA-CUCUUCCUGGcaccAGAGCg -3' miRNA: 3'- -CGCAC----------UGGUaGAGAGGGACCc---UCUUG- -5' |
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| 31754 | 3' | -54.8 | NC_006938.1 | + | 6727 | 0.68 | 0.640338 |
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Target: 5'- -aGUG-CCGUCUCacCCCUcGGGAcGAGCu -3' miRNA: 3'- cgCACuGGUAGAGa-GGGA-CCCU-CUUG- -5' |
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| 31754 | 3' | -54.8 | NC_006938.1 | + | 23933 | 0.69 | 0.574937 |
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Target: 5'- uCGUGACCGUCUCgccugCCUGGGcucGGCu -3' miRNA: 3'- cGCACUGGUAGAGag---GGACCCuc-UUG- -5' |
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| 31754 | 3' | -54.8 | NC_006938.1 | + | 43403 | 0.71 | 0.49055 |
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Target: 5'- cGCGaUGAUCGUCUCUggcaggccucCCCgUGGGAGGc- -3' miRNA: 3'- -CGC-ACUGGUAGAGA----------GGG-ACCCUCUug -5' |
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| 31754 | 3' | -54.8 | NC_006938.1 | + | 32734 | 0.78 | 0.188625 |
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Target: 5'- cGCGUGGCCAUCaagggcacgaUCUGGGAGAACa -3' miRNA: 3'- -CGCACUGGUAGagag------GGACCCUCUUG- -5' |
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| 31754 | 3' | -54.8 | NC_006938.1 | + | 29229 | 1.12 | 0.000814 |
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Target: 5'- gGCGUGACCAUCUCUCCCUGGGAGAACg -3' miRNA: 3'- -CGCACUGGUAGAGAGGGACCCUCUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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