Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 31754 | 5' | -56.2 | NC_006938.1 | + | 39197 | 0.7 | 0.430341 |
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Target: 5'- -uGCACUCCCGCguGGAGUGGucucuGCaCCCUg -3' miRNA: 3'- cuUGUGAGGGUG--CCUCAUC-----UGcGGGA- -5' |
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| 31754 | 5' | -56.2 | NC_006938.1 | + | 7152 | 0.68 | 0.562183 |
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Target: 5'- cGAGCGCUCCCGCuGGAGacGGCGaauggCCg -3' miRNA: 3'- -CUUGUGAGGGUG-CCUCauCUGCg----GGa -5' |
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| 31754 | 5' | -56.2 | NC_006938.1 | + | 48155 | 0.66 | 0.691819 |
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Target: 5'- cGACGgUCCUACgcuguGGAGcGGACGCUCg -3' miRNA: 3'- cUUGUgAGGGUG-----CCUCaUCUGCGGGa -5' |
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| 31754 | 5' | -56.2 | NC_006938.1 | + | 46958 | 0.66 | 0.723644 |
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Target: 5'- cGGACACUCcgccgaCCGCcgacGAGUGGGCGCgCg -3' miRNA: 3'- -CUUGUGAG------GGUGc---CUCAUCUGCGgGa -5' |
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| 31754 | 5' | -56.2 | NC_006938.1 | + | 20804 | 0.72 | 0.375857 |
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Target: 5'- cGGACGCUgCCCuCGGuGGUGGcauCGCCCUg -3' miRNA: 3'- -CUUGUGA-GGGuGCC-UCAUCu--GCGGGA- -5' |
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| 31754 | 5' | -56.2 | NC_006938.1 | + | 60803 | 0.68 | 0.59442 |
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Target: 5'- -cGCGCaggaUCCCGCcGAGUAGAC-CCCg -3' miRNA: 3'- cuUGUG----AGGGUGcCUCAUCUGcGGGa -5' |
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| 31754 | 5' | -56.2 | NC_006938.1 | + | 41282 | 0.67 | 0.659489 |
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Target: 5'- uGAGCGCUCCCggcgACGGccuGGcAGGCgaaGCCCUu -3' miRNA: 3'- -CUUGUGAGGG----UGCC---UCaUCUG---CGGGA- -5' |
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| 31754 | 5' | -56.2 | NC_006938.1 | + | 52014 | 0.67 | 0.659489 |
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Target: 5'- cGAACACgacgcgacgCCCACGGAaguacugcuuGUGGAUGUCg- -3' miRNA: 3'- -CUUGUGa--------GGGUGCCU----------CAUCUGCGGga -5' |
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| 31754 | 5' | -56.2 | NC_006938.1 | + | 20146 | 0.66 | 0.691819 |
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Target: 5'- -cGC-CUCCCACGGGG-AGGCcuGCCa- -3' miRNA: 3'- cuUGuGAGGGUGCCUCaUCUG--CGGga -5' |
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| 31754 | 5' | -56.2 | NC_006938.1 | + | 29272 | 1.08 | 0.001176 |
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Target: 5'- gGAACACUCCCACGGAGUAGACGCCCUg -3' miRNA: 3'- -CUUGUGAGGGUGCCUCAUCUGCGGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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