Results 1 - 11 of 11 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31756 | 3' | -56.7 | NC_006938.1 | + | 21146 | 0.66 | 0.646796 |
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Target: 5'- -cGUCGCuccGGCCGCgUcgGCGAAGa- -3' miRNA: 3'- uuCAGCGu--CCGGCGgGaaCGCUUUgu -5' |
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| 31756 | 3' | -56.7 | NC_006938.1 | + | 9019 | 0.66 | 0.613806 |
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Target: 5'- ----aGCAGGCCGCCgCcaccaUGCGGAugGg -3' miRNA: 3'- uucagCGUCCGGCGG-Ga----ACGCUUugU- -5' |
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| 31756 | 3' | -56.7 | NC_006938.1 | + | 28950 | 0.67 | 0.591877 |
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Target: 5'- gAGGcUCG-AGGCCGCuggCCUggGCGGGACAg -3' miRNA: 3'- -UUC-AGCgUCCGGCG---GGAa-CGCUUUGU- -5' |
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| 31756 | 3' | -56.7 | NC_006938.1 | + | 8151 | 0.67 | 0.591877 |
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Target: 5'- gAAGcC-CAGGCCGCCUgcgGCGAGcGCGc -3' miRNA: 3'- -UUCaGcGUCCGGCGGGaa-CGCUU-UGU- -5' |
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| 31756 | 3' | -56.7 | NC_006938.1 | + | 45037 | 0.67 | 0.591877 |
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Target: 5'- uAGUCGaGGGCCGCCCa---GAGGCc -3' miRNA: 3'- uUCAGCgUCCGGCGGGaacgCUUUGu -5' |
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| 31756 | 3' | -56.7 | NC_006938.1 | + | 42537 | 0.67 | 0.537788 |
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Target: 5'- ---cCaCGGGCCGCCUggGCGGAACc -3' miRNA: 3'- uucaGcGUCCGGCGGGaaCGCUUUGu -5' |
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| 31756 | 3' | -56.7 | NC_006938.1 | + | 7908 | 0.68 | 0.506147 |
|
Target: 5'- --cUCcCAGGCCGCCCUUGCc----- -3' miRNA: 3'- uucAGcGUCCGGCGGGAACGcuuugu -5' |
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| 31756 | 3' | -56.7 | NC_006938.1 | + | 3293 | 0.68 | 0.495776 |
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Target: 5'- cAGUUGCAGGUCGCCU--GUGAGGu- -3' miRNA: 3'- uUCAGCGUCCGGCGGGaaCGCUUUgu -5' |
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| 31756 | 3' | -56.7 | NC_006938.1 | + | 1888 | 0.68 | 0.475329 |
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Target: 5'- gAGGUC-CGGGCCGCgCCgc-CGAAGCGu -3' miRNA: 3'- -UUCAGcGUCCGGCG-GGaacGCUUUGU- -5' |
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| 31756 | 3' | -56.7 | NC_006938.1 | + | 3748 | 0.7 | 0.4167 |
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Target: 5'- -uGUCGUacaccAGGCCGUCUUUG-GAAGCAa -3' miRNA: 3'- uuCAGCG-----UCCGGCGGGAACgCUUUGU- -5' |
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| 31756 | 3' | -56.7 | NC_006938.1 | + | 27091 | 1.06 | 0.001072 |
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Target: 5'- cAAGUCGCAGGCCGCCCUUGCGAAACAg -3' miRNA: 3'- -UUCAGCGUCCGGCGGGAACGCUUUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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