Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31758 | 3' | -51.1 | NC_006938.1 | + | 15822 | 0.66 | 0.916075 |
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Target: 5'- gCACgaGGCCAgaGGUCUGGAUCUCg--- -3' miRNA: 3'- -GUGg-UCGGUa-CCAGAUUUAGAGgaca -5' |
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| 31758 | 3' | -51.1 | NC_006938.1 | + | 10979 | 0.67 | 0.888053 |
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Target: 5'- aCAUCGGCCG-GGUCUGGGgagCcauggCCUGUc -3' miRNA: 3'- -GUGGUCGGUaCCAGAUUUa--Ga----GGACA- -5' |
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| 31758 | 3' | -51.1 | NC_006938.1 | + | 32036 | 0.67 | 0.855594 |
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Target: 5'- uCACCGGCCAaGGUCgagGAcAUCgaCCUGc -3' miRNA: 3'- -GUGGUCGGUaCCAGa--UU-UAGa-GGACa -5' |
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| 31758 | 3' | -51.1 | NC_006938.1 | + | 58114 | 0.69 | 0.776278 |
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Target: 5'- cCACCAGCCuggaGGUCUucaugcaggucggcGAgcuGUCUUCUGUg -3' miRNA: 3'- -GUGGUCGGua--CCAGA--------------UU---UAGAGGACA- -5' |
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| 31758 | 3' | -51.1 | NC_006938.1 | + | 25675 | 0.69 | 0.75846 |
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Target: 5'- aCGCgGGCCAccagugUGGgcgcuucucCUGGAUCUCCUGg -3' miRNA: 3'- -GUGgUCGGU------ACCa--------GAUUUAGAGGACa -5' |
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| 31758 | 3' | -51.1 | NC_006938.1 | + | 2769 | 0.71 | 0.69281 |
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Target: 5'- aCACCAGCCggugcgacaGUGGcCgcguuGUCUCCUGc -3' miRNA: 3'- -GUGGUCGG---------UACCaGauu--UAGAGGACa -5' |
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| 31758 | 3' | -51.1 | NC_006938.1 | + | 4835 | 0.73 | 0.539438 |
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Target: 5'- gCACCGGCCgaggaguucggcgagGUGGUCgag--CUCCUGUc -3' miRNA: 3'- -GUGGUCGG---------------UACCAGauuuaGAGGACA- -5' |
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| 31758 | 3' | -51.1 | NC_006938.1 | + | 25319 | 1.07 | 0.003759 |
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Target: 5'- gCACCAGCCAUGGUCUAAAUCUCCUGUu -3' miRNA: 3'- -GUGGUCGGUACCAGAUUUAGAGGACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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