Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31758 | 5' | -60.6 | NC_006938.1 | + | 47262 | 0.66 | 0.438475 |
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Target: 5'- aGAGcGGGuGCUUCgCGgGCCAggGGCUg -3' miRNA: 3'- aCUC-UCCuCGGAG-GCgCGGUa-CCGAa -5' |
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| 31758 | 5' | -60.6 | NC_006938.1 | + | 29153 | 0.66 | 0.419782 |
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Target: 5'- -aAGAGGGGCCUCgGCcgagucauauGCCAccgcUGGCc- -3' miRNA: 3'- acUCUCCUCGGAGgCG----------CGGU----ACCGaa -5' |
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| 31758 | 5' | -60.6 | NC_006938.1 | + | 38983 | 0.66 | 0.392669 |
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Target: 5'- aGGGAGGAgaaGCCgcgaGCGCgCAUGGCc- -3' miRNA: 3'- aCUCUCCU---CGGagg-CGCG-GUACCGaa -5' |
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| 31758 | 5' | -60.6 | NC_006938.1 | + | 4152 | 0.68 | 0.326161 |
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Target: 5'- cGAGAGGAGCUcaUG-GCCAUGGUc- -3' miRNA: 3'- aCUCUCCUCGGagGCgCGGUACCGaa -5' |
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| 31758 | 5' | -60.6 | NC_006938.1 | + | 33045 | 0.68 | 0.31846 |
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Target: 5'- -uGGAGGAG-CUCCGU-CCGUGGCg- -3' miRNA: 3'- acUCUCCUCgGAGGCGcGGUACCGaa -5' |
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| 31758 | 5' | -60.6 | NC_006938.1 | + | 61560 | 0.69 | 0.255297 |
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Target: 5'- cUGAGAGGA-CCUCCacgaucagcGCGCCAUaGGUg- -3' miRNA: 3'- -ACUCUCCUcGGAGG---------CGCGGUA-CCGaa -5' |
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| 31758 | 5' | -60.6 | NC_006938.1 | + | 13497 | 0.7 | 0.224863 |
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Target: 5'- cGGGAccuguGGAGCCacCCGCGCCG-GGCa- -3' miRNA: 3'- aCUCU-----CCUCGGa-GGCGCGGUaCCGaa -5' |
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| 31758 | 5' | -60.6 | NC_006938.1 | + | 25355 | 1.05 | 0.000521 |
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Target: 5'- cUGAGAGGAGCCUCCGCGCCAUGGCUUg -3' miRNA: 3'- -ACUCUCCUCGGAGGCGCGGUACCGAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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