Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 31768 | 5' | -54.7 | NC_006938.1 | + | 2000 | 0.68 | 0.678733 |
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Target: 5'- cGCCUgAGCgCGAGGAU-ACAgagugGUCGGc -3' miRNA: 3'- -CGGGgUCG-GCUCCUAcUGUaa---CAGCU- -5' |
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| 31768 | 5' | -54.7 | NC_006938.1 | + | 43310 | 0.68 | 0.700277 |
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Target: 5'- gGUCCCAGCCGAacaGGGcUGGC-UUGaCGGa -3' miRNA: 3'- -CGGGGUCGGCU---CCU-ACUGuAACaGCU- -5' |
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| 31768 | 5' | -54.7 | NC_006938.1 | + | 29321 | 0.67 | 0.732076 |
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Target: 5'- cGCCUC-GCCGAGGAUGAgCAcg--CGGu -3' miRNA: 3'- -CGGGGuCGGCUCCUACU-GUaacaGCU- -5' |
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| 31768 | 5' | -54.7 | NC_006938.1 | + | 55594 | 0.67 | 0.732076 |
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Target: 5'- -gCCgAGCaCGAGGAUGGgGacGUCGAa -3' miRNA: 3'- cgGGgUCG-GCUCCUACUgUaaCAGCU- -5' |
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| 31768 | 5' | -54.7 | NC_006938.1 | + | 28779 | 0.67 | 0.749721 |
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Target: 5'- gGCCCCugugauggcaaucuGGuuGAGGGUGGCAguccgGgccgCGAc -3' miRNA: 3'- -CGGGG--------------UCggCUCCUACUGUaa---Ca---GCU- -5' |
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| 31768 | 5' | -54.7 | NC_006938.1 | + | 40316 | 0.66 | 0.802307 |
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Target: 5'- cGCCUCGGUCGccgaccacucgaAGGGuccgcccaUGACGUcGUCGAa -3' miRNA: 3'- -CGGGGUCGGC------------UCCU--------ACUGUAaCAGCU- -5' |
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| 31768 | 5' | -54.7 | NC_006938.1 | + | 14219 | 1.11 | 0.001096 |
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Target: 5'- uGCCCCAGCCGAGGAUGACAUUGUCGAu -3' miRNA: 3'- -CGGGGUCGGCUCCUACUGUAACAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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