Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 31769 | 3' | -65 | NC_006938.1 | + | 15777 | 0.66 | 0.310639 |
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Target: 5'- --aGCCgacgacuugaacaUUGGCUCCGGCCG-GAgGAGa -3' miRNA: 3'- ugaCGG-------------GGCCGAGGCCGGCgCUgCUC- -5' |
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| 31769 | 3' | -65 | NC_006938.1 | + | 14282 | 0.66 | 0.293237 |
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Target: 5'- uGCUGCCCUGG-UCCGGgaugagagacuucuuCUugGCGAUGAGc -3' miRNA: 3'- -UGACGGGGCCgAGGCC---------------GG--CGCUGCUC- -5' |
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| 31769 | 3' | -65 | NC_006938.1 | + | 12819 | 0.66 | 0.318541 |
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Target: 5'- -gUGCCCCGGacgCCaGGCC-CuGCGAGa -3' miRNA: 3'- ugACGGGGCCga-GG-CCGGcGcUGCUC- -5' |
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| 31769 | 3' | -65 | NC_006938.1 | + | 12254 | 0.67 | 0.270804 |
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Target: 5'- aGCUGgaCCaCGGCcugCaCGGUCGUGGCGAGc -3' miRNA: 3'- -UGACg-GG-GCCGa--G-GCCGGCGCUGCUC- -5' |
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| 31769 | 3' | -65 | NC_006938.1 | + | 12088 | 1.08 | 0.000191 |
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Target: 5'- gACUGCCCCGGCUCCGGCCGCGACGAGg -3' miRNA: 3'- -UGACGGGGCCGAGGCCGGCGCUGCUC- -5' |
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| 31769 | 3' | -65 | NC_006938.1 | + | 9703 | 0.73 | 0.104186 |
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Target: 5'- gAUUGCCCCGGCUCCGaacugaGCCuGUGugGu- -3' miRNA: 3'- -UGACGGGGCCGAGGC------CGG-CGCugCuc -5' |
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| 31769 | 3' | -65 | NC_006938.1 | + | 9160 | 0.75 | 0.068144 |
|
Target: 5'- --gGCCUCGaCUCCGGCCGCGACa-- -3' miRNA: 3'- ugaCGGGGCcGAGGCCGGCGCUGcuc -5' |
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| 31769 | 3' | -65 | NC_006938.1 | + | 8134 | 0.68 | 0.228994 |
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Target: 5'- uCUGCCCCugGGCUCCuauggagacucGGCCGUGcacCGAu -3' miRNA: 3'- uGACGGGG--CCGAGG-----------CCGGCGCu--GCUc -5' |
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| 31769 | 3' | -65 | NC_006938.1 | + | 7899 | 0.66 | 0.318541 |
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Target: 5'- gGCUGCUC--GCUCCuGGUCGuUGACGAGu -3' miRNA: 3'- -UGACGGGgcCGAGG-CCGGC-GCUGCUC- -5' |
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| 31769 | 3' | -65 | NC_006938.1 | + | 4827 | 0.67 | 0.263848 |
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Target: 5'- -aUGaCCUGGCaCCGGCCGaggaguuCGGCGAGg -3' miRNA: 3'- ugACgGGGCCGaGGCCGGC-------GCUGCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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