Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31769 | 3' | -65 | NC_006938.1 | + | 29148 | 0.7 | 0.157212 |
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Target: 5'- gGCUGgUCUGGCUCCGGCacgauguCGC-ACGAGu -3' miRNA: 3'- -UGACgGGGCCGAGGCCG-------GCGcUGCUC- -5' |
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| 31769 | 3' | -65 | NC_006938.1 | + | 21267 | 0.7 | 0.149776 |
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Target: 5'- -aUGCCCUGGUU-CGGuCCGCGuCGAGc -3' miRNA: 3'- ugACGGGGCCGAgGCC-GGCGCuGCUC- -5' |
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| 31769 | 3' | -65 | NC_006938.1 | + | 23947 | 0.71 | 0.145993 |
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Target: 5'- cCUGCCUgGGCU-CGGCUGUGAUGGu -3' miRNA: 3'- uGACGGGgCCGAgGCCGGCGCUGCUc -5' |
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| 31769 | 3' | -65 | NC_006938.1 | + | 31019 | 0.71 | 0.135159 |
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Target: 5'- --aGCCgagaaagcaUGGUUCUGGCCGCGugGAGa -3' miRNA: 3'- ugaCGGg--------GCCGAGGCCGGCGCugCUC- -5' |
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| 31769 | 3' | -65 | NC_006938.1 | + | 9703 | 0.73 | 0.104186 |
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Target: 5'- gAUUGCCCCGGCUCCGaacugaGCCuGUGugGu- -3' miRNA: 3'- -UGACGGGGCCGAGGC------CGG-CGCugCuc -5' |
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| 31769 | 3' | -65 | NC_006938.1 | + | 46583 | 0.74 | 0.082138 |
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Target: 5'- cACgGCCCCGcGCUCCccaGCUGCcGACGAGg -3' miRNA: 3'- -UGaCGGGGC-CGAGGc--CGGCG-CUGCUC- -5' |
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| 31769 | 3' | -65 | NC_006938.1 | + | 48392 | 0.74 | 0.078715 |
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Target: 5'- gGCUGCUcguacaccguggucaCCGGCUCCGGa-GCGACGAc -3' miRNA: 3'- -UGACGG---------------GGCCGAGGCCggCGCUGCUc -5' |
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| 31769 | 3' | -65 | NC_006938.1 | + | 9160 | 0.75 | 0.068144 |
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Target: 5'- --gGCCUCGaCUCCGGCCGCGACa-- -3' miRNA: 3'- ugaCGGGGCcGAGGCCGGCGCUGcuc -5' |
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| 31769 | 3' | -65 | NC_006938.1 | + | 48337 | 0.75 | 0.066343 |
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Target: 5'- cCUGaaCCCCGGCUCgggCGGCgGUGACGAGc -3' miRNA: 3'- uGAC--GGGGCCGAG---GCCGgCGCUGCUC- -5' |
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| 31769 | 3' | -65 | NC_006938.1 | + | 12088 | 1.08 | 0.000191 |
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Target: 5'- gACUGCCCCGGCUCCGGCCGCGACGAGg -3' miRNA: 3'- -UGACGGGGCCGAGGCCGGCGCUGCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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