Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31771 | 5' | -60.5 | NC_006938.1 | + | 12772 | 0.67 | 0.396103 |
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Target: 5'- gACCGcuGGGAGCUGCUcCAggacGGAGC-GGa -3' miRNA: 3'- -UGGC--UCCUCGACGAcGU----CCUCGaCCa -5' |
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| 31771 | 5' | -60.5 | NC_006938.1 | + | 51793 | 0.68 | 0.345168 |
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Target: 5'- cAUCGAGGcgaccaaccGGCUGCgcgacacagaGCAGGAGCUGc- -3' miRNA: 3'- -UGGCUCC---------UCGACGa---------CGUCCUCGACca -5' |
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| 31771 | 5' | -60.5 | NC_006938.1 | + | 41618 | 0.68 | 0.314669 |
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Target: 5'- gGCCaGGGGAGCgccgcucuucaacccGCUGCAGGucuCUGGUc -3' miRNA: 3'- -UGG-CUCCUCGa--------------CGACGUCCuc-GACCA- -5' |
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| 31771 | 5' | -60.5 | NC_006938.1 | + | 20334 | 0.69 | 0.299109 |
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Target: 5'- gGCUGGcuGGAGCUGaaGCAGGuggacAGCUGGc -3' miRNA: 3'- -UGGCU--CCUCGACgaCGUCC-----UCGACCa -5' |
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| 31771 | 5' | -60.5 | NC_006938.1 | + | 31818 | 0.69 | 0.271133 |
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Target: 5'- gACUGGcucGAGCUGCUggaGCGGGAGCUGu- -3' miRNA: 3'- -UGGCUc--CUCGACGA---CGUCCUCGACca -5' |
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| 31771 | 5' | -60.5 | NC_006938.1 | + | 54059 | 0.69 | 0.271133 |
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Target: 5'- --gGAGGGGCUGCUcgccGCAGGGaUUGGUg -3' miRNA: 3'- uggCUCCUCGACGA----CGUCCUcGACCA- -5' |
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| 31771 | 5' | -60.5 | NC_006938.1 | + | 22999 | 0.73 | 0.149298 |
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Target: 5'- aACCcgGAGGAcgGCUGgUcGCGGGAGCUGGa -3' miRNA: 3'- -UGG--CUCCU--CGACgA-CGUCCUCGACCa -5' |
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| 31771 | 5' | -60.5 | NC_006938.1 | + | 11407 | 1.06 | 0.000502 |
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Target: 5'- cACCGAGGAGCUGCUGCAGGAGCUGGUu -3' miRNA: 3'- -UGGCUCCUCGACGACGUCCUCGACCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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