Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31778 | 5' | -54.1 | NC_006938.1 | + | 61688 | 0.66 | 0.805761 |
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Target: 5'- -cGGCCCggaCCUcugUGACG-ACGCUgaAUGg -3' miRNA: 3'- cuCCGGGa--GGA---ACUGCuUGCGA--UACa -5' |
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| 31778 | 5' | -54.1 | NC_006938.1 | + | 25975 | 0.66 | 0.805761 |
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Target: 5'- cGGGGCCgUCUgggUGGCGAugGUgcccUGg -3' miRNA: 3'- -CUCCGGgAGGa--ACUGCUugCGau--ACa -5' |
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| 31778 | 5' | -54.1 | NC_006938.1 | + | 40158 | 0.66 | 0.805761 |
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Target: 5'- aGAGGgCCUUCggGGCGAGCuugacgGUUGUGUc -3' miRNA: 3'- -CUCCgGGAGGaaCUGCUUG------CGAUACA- -5' |
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| 31778 | 5' | -54.1 | NC_006938.1 | + | 13965 | 0.66 | 0.776199 |
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Target: 5'- gGAGGCa-UCCUcGGCGAGCgGCUGUa- -3' miRNA: 3'- -CUCCGggAGGAaCUGCUUG-CGAUAca -5' |
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| 31778 | 5' | -54.1 | NC_006938.1 | + | 53802 | 0.67 | 0.713308 |
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Target: 5'- -cGGCCg-CgUUGGCGAGgGCUGUGa -3' miRNA: 3'- cuCCGGgaGgAACUGCUUgCGAUACa -5' |
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| 31778 | 5' | -54.1 | NC_006938.1 | + | 17471 | 0.68 | 0.679509 |
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Target: 5'- cAGGuCCCUCCgugcgGACGGGCggucacaucgcugGCUAUGg -3' miRNA: 3'- cUCC-GGGAGGaa---CUGCUUG-------------CGAUACa -5' |
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| 31778 | 5' | -54.1 | NC_006938.1 | + | 59856 | 0.69 | 0.625315 |
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Target: 5'- gGAGGCCUcgUCCagGAUGAACGCg---- -3' miRNA: 3'- -CUCCGGG--AGGaaCUGCUUGCGauaca -5' |
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| 31778 | 5' | -54.1 | NC_006938.1 | + | 2014 | 0.78 | 0.200817 |
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Target: 5'- gGAGGUCCUCUcaGACGGACGCUgGUGg -3' miRNA: 3'- -CUCCGGGAGGaaCUGCUUGCGA-UACa -5' |
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| 31778 | 5' | -54.1 | NC_006938.1 | + | 2215 | 1.08 | 0.001598 |
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Target: 5'- cGAGGCCCUCCUUGACGAACGCUAUGUg -3' miRNA: 3'- -CUCCGGGAGGAACUGCUUGCGAUACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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