Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31780 | 3' | -53.2 | NC_006938.1 | + | 51477 | 0.7 | 0.621865 |
Target: 5'- ---cGGGUUCUCGUCggcGGUGAC-GCg- -3' miRNA: 3'- caaaCCCGAGAGCAG---UCACUGuCGac -5' |
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31780 | 3' | -53.2 | NC_006938.1 | + | 45094 | 0.68 | 0.721642 |
Target: 5'- ---gGGGCgaugucCUCGUCGcggGACGGCUGc -3' miRNA: 3'- caaaCCCGa-----GAGCAGUca-CUGUCGAC- -5' |
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31780 | 3' | -53.2 | NC_006938.1 | + | 25922 | 0.68 | 0.710767 |
Target: 5'- --cUGGGCggcaaaCUCGUCAcgcuccuuGUGAgGGCUGc -3' miRNA: 3'- caaACCCGa-----GAGCAGU--------CACUgUCGAC- -5' |
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31780 | 3' | -53.2 | NC_006938.1 | + | 25232 | 0.67 | 0.774397 |
Target: 5'- ---cGGGCcuUCUCuUCAGUGAgCuGCUGg -3' miRNA: 3'- caaaCCCG--AGAGcAGUCACU-GuCGAC- -5' |
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31780 | 3' | -53.2 | NC_006938.1 | + | 23952 | 0.69 | 0.678819 |
Target: 5'- --cUGGGCUCggcugugauggucagCGUgcCAGUGGCGGCg- -3' miRNA: 3'- caaACCCGAGa--------------GCA--GUCACUGUCGac -5' |
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31780 | 3' | -53.2 | NC_006938.1 | + | 1241 | 1.08 | 0.002018 |
Target: 5'- uGUUUGGGCUCUCGUCAGUGACAGCUGu -3' miRNA: 3'- -CAAACCCGAGAGCAGUCACUGUCGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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