Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31780 | 5' | -59.2 | NC_006938.1 | + | 9865 | 0.7 | 0.341742 |
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Target: 5'- cCUAUCagGCcucCGUCGAgcuGGCCCAGGAGa -3' miRNA: 3'- aGGUGGa-CGc--GCAGCU---UCGGGUCCUC- -5' |
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| 31780 | 5' | -59.2 | NC_006938.1 | + | 34750 | 0.7 | 0.326098 |
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Target: 5'- cUgACCgUGCGCGUCGcGGCCCGGa-- -3' miRNA: 3'- aGgUGG-ACGCGCAGCuUCGGGUCcuc -5' |
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| 31780 | 5' | -59.2 | NC_006938.1 | + | 15367 | 0.7 | 0.318477 |
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Target: 5'- --gACCUGCGUGUUGucGCCCguGGGAu -3' miRNA: 3'- aggUGGACGCGCAGCuuCGGG--UCCUc -5' |
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| 31780 | 5' | -59.2 | NC_006938.1 | + | 43426 | 0.72 | 0.248998 |
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Target: 5'- -gCGCCUcgucccaGCGCugguuGUCgGAGGCCCAGGAGa -3' miRNA: 3'- agGUGGA-------CGCG-----CAG-CUUCGGGUCCUC- -5' |
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| 31780 | 5' | -59.2 | NC_006938.1 | + | 26412 | 0.73 | 0.20909 |
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Target: 5'- aUCCACgUGCGCaUCGAGGUgaAGGAGu -3' miRNA: 3'- -AGGUGgACGCGcAGCUUCGggUCCUC- -5' |
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| 31780 | 5' | -59.2 | NC_006938.1 | + | 39661 | 0.74 | 0.183726 |
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Target: 5'- gUCCGCCcGCGCGUCGAucacGCCCuuGAa -3' miRNA: 3'- -AGGUGGaCGCGCAGCUu---CGGGucCUc -5' |
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| 31780 | 5' | -59.2 | NC_006938.1 | + | 57672 | 0.74 | 0.169845 |
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Target: 5'- cUCCACCuUGCGC-UCGAacAGUCCGGGGa -3' miRNA: 3'- -AGGUGG-ACGCGcAGCU--UCGGGUCCUc -5' |
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| 31780 | 5' | -59.2 | NC_006938.1 | + | 1275 | 1.08 | 0.000608 |
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Target: 5'- gUCCACCUGCGCGUCGAAGCCCAGGAGc -3' miRNA: 3'- -AGGUGGACGCGCAGCUUCGGGUCCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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