Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31788 | 3' | -49.2 | NC_006938.1 | + | 53914 | 0.66 | 0.958781 |
Target: 5'- -cUGGAGCGCggCCUCGGuGCC-CAgGg -3' miRNA: 3'- caACUUUGCGuaGGAGUU-CGGuGUgU- -5' |
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31788 | 3' | -49.2 | NC_006938.1 | + | 62650 | 0.66 | 0.962761 |
Target: 5'- -aUGAGACGgAacUCCUCGGccGCCgACGCGu -3' miRNA: 3'- caACUUUGCgU--AGGAGUU--CGG-UGUGU- -5' |
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31788 | 3' | -49.2 | NC_006938.1 | + | 34316 | 0.66 | 0.962761 |
Target: 5'- -aUGAGcACGCGguacUUCUCcuGCCACACc -3' miRNA: 3'- caACUU-UGCGU----AGGAGuuCGGUGUGu -5' |
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31788 | 3' | -49.2 | NC_006938.1 | + | 59677 | 0.7 | 0.808167 |
Target: 5'- cGUUGAAggugcuugccGCGCG-CUUCuGAGCCGCGCGu -3' miRNA: 3'- -CAACUU----------UGCGUaGGAG-UUCGGUGUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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