Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31788 | 5' | -55.4 | NC_006938.1 | + | 30731 | 0.67 | 0.655103 |
Target: 5'- -aCCUGAgccAGGGucgcggcucGGGCGCGUUCGugCUc -3' miRNA: 3'- caGGACU---UCUU---------CCUGCGCGAGCugGA- -5' |
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31788 | 5' | -55.4 | NC_006938.1 | + | 48445 | 0.66 | 0.751066 |
Target: 5'- cUCC-GAc--AGGACGCGCUCGucACCg -3' miRNA: 3'- cAGGaCUucuUCCUGCGCGAGC--UGGa -5' |
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31788 | 5' | -55.4 | NC_006938.1 | + | 4388 | 0.76 | 0.231814 |
Target: 5'- cUCCuUGAAGGuguGGACGCGCUCcACCg -3' miRNA: 3'- cAGG-ACUUCUu--CCUGCGCGAGcUGGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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