Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31792 | 3' | -52.4 | NC_006938.1 | + | 10223 | 0.71 | 0.632475 |
Target: 5'- cGCGGaCaCCGUagUCGUCcACCcGCUCCg -3' miRNA: 3'- aCGCUaG-GGCA--AGUAGuUGGaCGAGG- -5' |
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31792 | 3' | -52.4 | NC_006938.1 | + | 6231 | 0.72 | 0.566192 |
Target: 5'- aGCGGUCCgCGUccUCGU-AGCCcgugGCUCCg -3' miRNA: 3'- aCGCUAGG-GCA--AGUAgUUGGa---CGAGG- -5' |
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31792 | 3' | -52.4 | NC_006938.1 | + | 32906 | 0.72 | 0.566192 |
Target: 5'- cGCGGccagCuuGgcggggUUGUCGGCCUGCUCCu -3' miRNA: 3'- aCGCUa---GggCa-----AGUAGUUGGACGAGG- -5' |
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31792 | 3' | -52.4 | NC_006938.1 | + | 62564 | 0.75 | 0.431126 |
Target: 5'- gGCGGccgaggagUUCCGUcUCAUCGucccgaccagugGCCUGCUCCg -3' miRNA: 3'- aCGCU--------AGGGCA-AGUAGU------------UGGACGAGG- -5' |
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31792 | 3' | -52.4 | NC_006938.1 | + | 4211 | 0.76 | 0.375406 |
Target: 5'- cGcCGAgcUCCCcaUCGUCAACgUGCUCCg -3' miRNA: 3'- aC-GCU--AGGGcaAGUAGUUGgACGAGG- -5' |
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31792 | 3' | -52.4 | NC_006938.1 | + | 50381 | 0.81 | 0.183022 |
Target: 5'- aGCGGaggccaccgCUCGUcgCAUCAACCUGCUCCg -3' miRNA: 3'- aCGCUa--------GGGCAa-GUAGUUGGACGAGG- -5' |
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31792 | 3' | -52.4 | NC_006938.1 | + | 6175 | 1.13 | 0.001194 |
Target: 5'- uUGCGAUCCCGUUCAUCAACCUGCUCCa -3' miRNA: 3'- -ACGCUAGGGCAAGUAGUUGGACGAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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