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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31794 | 3' | -55.2 | NC_006938.1 | + | 5772 | 0.66 | 0.771367 |
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Target: 5'- gGCCGc-CGUGaCUGUGAcCUCCUcCAGu -3' miRNA: 3'- -CGGCucGCGC-GACACUaGAGGAaGUC- -5' |
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| 31794 | 3' | -55.2 | NC_006938.1 | + | 28593 | 0.66 | 0.730288 |
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Target: 5'- gGCCGAGCgGCGCUugccacGgagGAgcucCUCCUUCu- -3' miRNA: 3'- -CGGCUCG-CGCGA------Ca--CUa---GAGGAAGuc -5' |
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| 31794 | 3' | -55.2 | NC_006938.1 | + | 5736 | 0.67 | 0.709124 |
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Target: 5'- cUCGAGgGUGUUGggaaUGGUCUCCU-CGGa -3' miRNA: 3'- cGGCUCgCGCGAC----ACUAGAGGAaGUC- -5' |
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| 31794 | 3' | -55.2 | NC_006938.1 | + | 13894 | 0.67 | 0.665991 |
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Target: 5'- uGCCGAGUggggacgauGCGCU-UGAUCUUCUugucgagcaccaUCAGg -3' miRNA: 3'- -CGGCUCG---------CGCGAcACUAGAGGA------------AGUC- -5' |
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| 31794 | 3' | -55.2 | NC_006938.1 | + | 6506 | 1.1 | 0.001085 |
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Target: 5'- uGCCGAGCGCGCUGUGAUCUCCUUCAGc -3' miRNA: 3'- -CGGCUCGCGCGACACUAGAGGAAGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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