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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31794 | 5' | -45.7 | NC_006938.1 | + | 6540 | 1.12 | 0.006695 |
Target: 5'- aAGCUGAAGAAGUAUCGUCAGUUCUUCa -3' miRNA: 3'- -UCGACUUCUUCAUAGCAGUCAAGAAG- -5' |
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31794 | 5' | -45.7 | NC_006938.1 | + | 37481 | 0.69 | 0.98305 |
Target: 5'- uGUUGAGGGAG-GUCGUCAGgcgaUCgacgUCu -3' miRNA: 3'- uCGACUUCUUCaUAGCAGUCa---AGa---AG- -5' |
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31794 | 5' | -45.7 | NC_006938.1 | + | 46668 | 0.68 | 0.988767 |
Target: 5'- cAGCUGggGAGcgcggggccGUGUCGccucucCAGggCUUCg -3' miRNA: 3'- -UCGACuuCUU---------CAUAGCa-----GUCaaGAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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