Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31795 | 5' | -63.8 | NC_006938.1 | + | 6237 | 0.66 | 0.35423 |
Target: 5'- gUUCaGAGCGGUCCGcGUccucguagcccgugGCUCCgGCa -3' miRNA: 3'- -AGGcCUCGCCAGGCuCG--------------CGAGGgCGa -5' |
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31795 | 5' | -63.8 | NC_006938.1 | + | 25766 | 0.66 | 0.348738 |
Target: 5'- cUCCGuGGUGGUCUGGGCcacCUCCCcgaGCg -3' miRNA: 3'- -AGGCcUCGCCAGGCUCGc--GAGGG---CGa -5' |
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31795 | 5' | -63.8 | NC_006938.1 | + | 17574 | 0.66 | 0.333384 |
Target: 5'- cCUGGAcGCGGcCCaGGUGCU-CCGCUa -3' miRNA: 3'- aGGCCU-CGCCaGGcUCGCGAgGGCGA- -5' |
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31795 | 5' | -63.8 | NC_006938.1 | + | 52556 | 0.69 | 0.234112 |
Target: 5'- gUCUGGAGCGGgaucucgCCGgucGGCGUcgUCaCCGCg -3' miRNA: 3'- -AGGCCUCGCCa------GGC---UCGCG--AG-GGCGa -5' |
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31795 | 5' | -63.8 | NC_006938.1 | + | 48344 | 0.69 | 0.233541 |
Target: 5'- cCCGGcucgggcGGCGGUgaCGAGCGCgUCCUGUc -3' miRNA: 3'- aGGCC-------UCGCCAg-GCUCGCG-AGGGCGa -5' |
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31795 | 5' | -63.8 | NC_006938.1 | + | 50425 | 0.7 | 0.20193 |
Target: 5'- -aUGGAGCGGUugaCCGuGCGCgcgCCaCGCUc -3' miRNA: 3'- agGCCUCGCCA---GGCuCGCGa--GG-GCGA- -5' |
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31795 | 5' | -63.8 | NC_006938.1 | + | 41026 | 0.71 | 0.165053 |
Target: 5'- aUCgCGGGGCGGgucgagCCGAGCuuggGCUUgCGCUg -3' miRNA: 3'- -AG-GCCUCGCCa-----GGCUCG----CGAGgGCGA- -5' |
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31795 | 5' | -63.8 | NC_006938.1 | + | 7139 | 1.07 | 0.000261 |
Target: 5'- gUCCGGAGCGGUCCGAGCGCUCCCGCUg -3' miRNA: 3'- -AGGCCUCGCCAGGCUCGCGAGGGCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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