Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31797 | 3' | -60 | NC_006938.1 | + | 38053 | 0.66 | 0.456336 |
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Target: 5'- ---cCUCGGCcgUGAACGGGUUGCucGCGc -3' miRNA: 3'- uucuGGGCCG--ACUUGCCCGACGu-CGU- -5' |
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| 31797 | 3' | -60 | NC_006938.1 | + | 15479 | 0.66 | 0.446674 |
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Target: 5'- cAGGAgCgGGCUGAcGCGGGCgGCAaggacguccccuGCAa -3' miRNA: 3'- -UUCUgGgCCGACU-UGCCCGaCGU------------CGU- -5' |
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| 31797 | 3' | -60 | NC_006938.1 | + | 7566 | 0.66 | 0.437127 |
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Target: 5'- -cGACCCGGCUcaGGGCcguguucuguuGGGCuccccacuucaUGCGGCGu -3' miRNA: 3'- uuCUGGGCCGA--CUUG-----------CCCG-----------ACGUCGU- -5' |
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| 31797 | 3' | -60 | NC_006938.1 | + | 46269 | 0.66 | 0.427698 |
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Target: 5'- cAGGCCCGcGCgcgGAAacuccagGGGCUGCgAGCc -3' miRNA: 3'- uUCUGGGC-CGa--CUUg------CCCGACG-UCGu -5' |
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| 31797 | 3' | -60 | NC_006938.1 | + | 2780 | 0.66 | 0.427698 |
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Target: 5'- cGAGcGCCCGGCgGGACaGGUUgGCGGUg -3' miRNA: 3'- -UUC-UGGGCCGaCUUGcCCGA-CGUCGu -5' |
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| 31797 | 3' | -60 | NC_006938.1 | + | 35811 | 0.67 | 0.41839 |
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Target: 5'- cAGGUCCaGCUGGACGGGCgacacGguGCGc -3' miRNA: 3'- uUCUGGGcCGACUUGCCCGa----CguCGU- -5' |
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| 31797 | 3' | -60 | NC_006938.1 | + | 49685 | 0.67 | 0.409207 |
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Target: 5'- gGAGGCauccuCGGC-GAGC-GGCUGUAGCAg -3' miRNA: 3'- -UUCUGg----GCCGaCUUGcCCGACGUCGU- -5' |
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| 31797 | 3' | -60 | NC_006938.1 | + | 40203 | 0.69 | 0.309356 |
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Target: 5'- --cACCCGGCUGAcCGGcGCUGUccGCGu -3' miRNA: 3'- uucUGGGCCGACUuGCC-CGACGu-CGU- -5' |
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| 31797 | 3' | -60 | NC_006938.1 | + | 46897 | 0.73 | 0.158558 |
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Target: 5'- gAGGAUCCGGaCgucGGCGGGCUGCAGgAu -3' miRNA: 3'- -UUCUGGGCC-Gac-UUGCCCGACGUCgU- -5' |
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| 31797 | 3' | -60 | NC_006938.1 | + | 7715 | 1.06 | 0.000534 |
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Target: 5'- cAAGACCCGGCUGAACGGGCUGCAGCAg -3' miRNA: 3'- -UUCUGGGCCGACUUGCCCGACGUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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