Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 31798 | 5' | -55.5 | NC_006938.1 | + | 61812 | 0.69 | 0.600233 |
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Target: 5'- cGCC-GUCCcc-GuCCAgGAGGAGGGa -3' miRNA: 3'- -CGGaCAGGuuaCuGGUgCUCCUCCCg -5' |
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| 31798 | 5' | -55.5 | NC_006938.1 | + | 51790 | 0.67 | 0.685608 |
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Target: 5'- aGCCUGUggCCAGUGGggagccgguugacUCGCGgagcccAGaGAGGGCg -3' miRNA: 3'- -CGGACA--GGUUACU-------------GGUGC------UC-CUCCCG- -5' |
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| 31798 | 5' | -55.5 | NC_006938.1 | + | 3841 | 0.66 | 0.729005 |
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Target: 5'- cGCCcgacgagcGUCuUggUGAUCuGCGAGGuGGGCg -3' miRNA: 3'- -CGGa-------CAG-GuuACUGG-UGCUCCuCCCG- -5' |
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| 31798 | 5' | -55.5 | NC_006938.1 | + | 22785 | 0.66 | 0.739379 |
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Target: 5'- -gCUGUUgGAggacaUGACCACGuGGAgguaguucGGGCa -3' miRNA: 3'- cgGACAGgUU-----ACUGGUGCuCCU--------CCCG- -5' |
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| 31798 | 5' | -55.5 | NC_006938.1 | + | 18274 | 0.76 | 0.239685 |
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Target: 5'- aGCUggUGUCC---GGCCGCGcGGAGGGCg -3' miRNA: 3'- -CGG--ACAGGuuaCUGGUGCuCCUCCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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