Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
318 | 3' | -54.7 | AC_000010.1 | + | 8563 | 0.66 | 0.642081 |
Target: 5'- cGCGcgCGGCGCCgacgugccacuGCCAGCcaugccgccggGGCCCg-- -3' miRNA: 3'- -CGCaaGUUGCGG-----------UGGUCG-----------CUGGGauc -5' |
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318 | 3' | -54.7 | AC_000010.1 | + | 6381 | 0.66 | 0.61933 |
Target: 5'- gGCGag-GugGCCACCAGUGuggaucuaaucACCCUGc -3' miRNA: 3'- -CGCaagUugCGGUGGUCGC-----------UGGGAUc -5' |
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318 | 3' | -54.7 | AC_000010.1 | + | 9354 | 0.66 | 0.596627 |
Target: 5'- cCGUUUGcccguGCGCCGCCGGCGucgccgcguGCCCc-- -3' miRNA: 3'- cGCAAGU-----UGCGGUGGUCGC---------UGGGauc -5' |
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318 | 3' | -54.7 | AC_000010.1 | + | 12503 | 0.67 | 0.562836 |
Target: 5'- gGCGUUgAACGCCuuccUCAGC-ACCCa-- -3' miRNA: 3'- -CGCAAgUUGCGGu---GGUCGcUGGGauc -5' |
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318 | 3' | -54.7 | AC_000010.1 | + | 17402 | 0.67 | 0.562836 |
Target: 5'- cGCGggaugCGACGCCACaggCGGCGGCgCg-- -3' miRNA: 3'- -CGCaa---GUUGCGGUG---GUCGCUGgGauc -5' |
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318 | 3' | -54.7 | AC_000010.1 | + | 5148 | 0.68 | 0.52958 |
Target: 5'- gGCGUcCAGCGCUGCCAGgG-UCCg-- -3' miRNA: 3'- -CGCAaGUUGCGGUGGUCgCuGGGauc -5' |
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318 | 3' | -54.7 | AC_000010.1 | + | 16132 | 0.7 | 0.386827 |
Target: 5'- gGUGcgCGACGCCACCAcCGGCCa--- -3' miRNA: 3'- -CGCaaGUUGCGGUGGUcGCUGGgauc -5' |
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318 | 3' | -54.7 | AC_000010.1 | + | 12550 | 1.11 | 0.000479 |
Target: 5'- gGCGUUCAACGCCACCAGCGACCCUAGa -3' miRNA: 3'- -CGCAAGUUGCGGUGGUCGCUGGGAUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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