Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 31800 | 3' | -53.7 | NC_006938.1 | + | 52227 | 0.67 | 0.769459 |
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Target: 5'- uGGA-GGCGGAGGGCuCUuuggugGAGGAGg -3' miRNA: 3'- uCCUgCCGCUUCUCGuGAug----UUCCUC- -5' |
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| 31800 | 3' | -53.7 | NC_006938.1 | + | 53496 | 0.68 | 0.695349 |
|
Target: 5'- cGGucuCGGUGAGG-GCACUGCuguccAGGAa -3' miRNA: 3'- uCCu--GCCGCUUCuCGUGAUGu----UCCUc -5' |
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| 31800 | 3' | -53.7 | NC_006938.1 | + | 55655 | 0.7 | 0.585084 |
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Target: 5'- cGGaGGCGGC-AAGGGCGgcCUGgGAGGAGg -3' miRNA: 3'- -UC-CUGCCGcUUCUCGU--GAUgUUCCUC- -5' |
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| 31800 | 3' | -53.7 | NC_006938.1 | + | 55850 | 0.66 | 0.827564 |
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Target: 5'- -cGACGGCGggGAGgaccaGCUGCc-GGAc -3' miRNA: 3'- ucCUGCCGCuuCUCg----UGAUGuuCCUc -5' |
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| 31800 | 3' | -53.7 | NC_006938.1 | + | 60145 | 0.66 | 0.827564 |
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Target: 5'- cAGGugGGCGcGGGGCAg-ACGAccauugucgccGGAGc -3' miRNA: 3'- -UCCugCCGCuUCUCGUgaUGUU-----------CCUC- -5' |
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| 31800 | 3' | -53.7 | NC_006938.1 | + | 61736 | 0.66 | 0.818339 |
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Target: 5'- uGGACGGgGAc-GGCgACUACcuGGAGu -3' miRNA: 3'- uCCUGCCgCUucUCG-UGAUGuuCCUC- -5' |
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| 31800 | 3' | -53.7 | NC_006938.1 | + | 63406 | 0.68 | 0.717006 |
|
Target: 5'- uGGACGGCGAGGucCGCU---GGGAc -3' miRNA: 3'- uCCUGCCGCUUCucGUGAuguUCCUc -5' |
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| 31800 | 3' | -53.7 | NC_006938.1 | + | 63498 | 0.73 | 0.438537 |
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Target: 5'- uGGGGCGGUGAcuGGcGCACguuCAAGGAc -3' miRNA: 3'- -UCCUGCCGCU--UCuCGUGau-GUUCCUc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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