Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31800 | 3' | -53.7 | NC_006938.1 | + | 14356 | 0.69 | 0.640318 |
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Target: 5'- cGGACcGCGAGGgcuuuGGCACUcGgAAGGAGg -3' miRNA: 3'- uCCUGcCGCUUC-----UCGUGA-UgUUCCUC- -5' |
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| 31800 | 3' | -53.7 | NC_006938.1 | + | 43912 | 0.7 | 0.607122 |
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Target: 5'- cAGGugGGUGugacggccguGAGCAUccACAGGGAGu -3' miRNA: 3'- -UCCugCCGCuu--------CUCGUGa-UGUUCCUC- -5' |
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| 31800 | 3' | -53.7 | NC_006938.1 | + | 55655 | 0.7 | 0.585084 |
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Target: 5'- cGGaGGCGGC-AAGGGCGgcCUGgGAGGAGg -3' miRNA: 3'- -UC-CUGCCGcUUCUCGU--GAUgUUCCUC- -5' |
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| 31800 | 3' | -53.7 | NC_006938.1 | + | 37916 | 0.71 | 0.51912 |
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Target: 5'- cAGGGCGGCGAGcGGGCGgUggccaggGCGGuGGAGg -3' miRNA: 3'- -UCCUGCCGCUU-CUCGUgA-------UGUU-CCUC- -5' |
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| 31800 | 3' | -53.7 | NC_006938.1 | + | 32411 | 0.72 | 0.485682 |
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Target: 5'- cGGACGGCGAGGAGCuGCUcuuccgccaguucaACAAGc-- -3' miRNA: 3'- uCCUGCCGCUUCUCG-UGA--------------UGUUCcuc -5' |
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| 31800 | 3' | -53.7 | NC_006938.1 | + | 63498 | 0.73 | 0.438537 |
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Target: 5'- uGGGGCGGUGAcuGGcGCACguuCAAGGAc -3' miRNA: 3'- -UCCUGCCGCU--UCuCGUGau-GUUCCUc -5' |
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| 31800 | 3' | -53.7 | NC_006938.1 | + | 27457 | 0.73 | 0.400549 |
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Target: 5'- cGGAgCGGcCGAGGAGCAC---GAGGAGa -3' miRNA: 3'- uCCU-GCC-GCUUCUCGUGaugUUCCUC- -5' |
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| 31800 | 3' | -53.7 | NC_006938.1 | + | 8836 | 1.08 | 0.001894 |
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Target: 5'- aAGGACGGCGAAGAGCACUACAAGGAGc -3' miRNA: 3'- -UCCUGCCGCUUCUCGUGAUGUUCCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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