Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31802 | 5' | -51.7 | NC_006938.1 | + | 17475 | 0.66 | 0.901407 |
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Target: 5'- cCAggaggGGAGgGUGgaCAUGUCUGGCGGc -3' miRNA: 3'- aGUa----CCUCaCGCa-GUAUAGACCGCUc -5' |
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| 31802 | 5' | -51.7 | NC_006938.1 | + | 11221 | 0.66 | 0.886922 |
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Target: 5'- -gGUGGGGgacggGCGggucgGUGUCgGGCGAGa -3' miRNA: 3'- agUACCUCa----CGCag---UAUAGaCCGCUC- -5' |
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| 31802 | 5' | -51.7 | NC_006938.1 | + | 25449 | 0.67 | 0.879272 |
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Target: 5'- aCGUGGAGcUGcCGUUcgAccagCUGGCGAa -3' miRNA: 3'- aGUACCUC-AC-GCAGuaUa---GACCGCUc -5' |
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| 31802 | 5' | -51.7 | NC_006938.1 | + | 11551 | 0.68 | 0.837201 |
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Target: 5'- cCGUGGGcGUcGCGUCGUGUUcgucgGGCGGc -3' miRNA: 3'- aGUACCU-CA-CGCAGUAUAGa----CCGCUc -5' |
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| 31802 | 5' | -51.7 | NC_006938.1 | + | 14519 | 0.69 | 0.75884 |
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Target: 5'- ---aGGAGUGCGUCc--UCUGGgaCGAGu -3' miRNA: 3'- aguaCCUCACGCAGuauAGACC--GCUC- -5' |
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| 31802 | 5' | -51.7 | NC_006938.1 | + | 37945 | 0.75 | 0.454249 |
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Target: 5'- -gGUGGAG-GCGUCGgcUCUGGCGcGa -3' miRNA: 3'- agUACCUCaCGCAGUauAGACCGCuC- -5' |
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| 31802 | 5' | -51.7 | NC_006938.1 | + | 9309 | 1.09 | 0.002657 |
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Target: 5'- uUCAUGGAGUGCGUCAUAUCUGGCGAGc -3' miRNA: 3'- -AGUACCUCACGCAGUAUAGACCGCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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