Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31808 | 3' | -66.7 | NC_006938.1 | + | 34138 | 0.73 | 0.079539 |
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Target: 5'- uGGUGCUCGgcCCGGaGGCCUggaCCCGCUg -3' miRNA: 3'- uCCGCGAGC--GGCC-CCGGAga-GGGCGG- -5' |
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| 31808 | 3' | -66.7 | NC_006938.1 | + | 55406 | 0.73 | 0.07157 |
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Target: 5'- cGGcGCGCUCGCCgcaGGcGGCCUgggcUUCUCGCCc -3' miRNA: 3'- -UC-CGCGAGCGG---CC-CCGGA----GAGGGCGG- -5' |
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| 31808 | 3' | -66.7 | NC_006938.1 | + | 26175 | 0.74 | 0.062684 |
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Target: 5'- cGGCuGCUUGCCGGGGaCUCcUCCGCg -3' miRNA: 3'- uCCG-CGAGCGGCCCCgGAGaGGGCGg -5' |
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| 31808 | 3' | -66.7 | NC_006938.1 | + | 25563 | 0.75 | 0.052014 |
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Target: 5'- aGGGCGUgaacgugucUCGCCuGGGCUUCUUcgCCGCCg -3' miRNA: 3'- -UCCGCG---------AGCGGcCCCGGAGAG--GGCGG- -5' |
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| 31808 | 3' | -66.7 | NC_006938.1 | + | 9594 | 0.79 | 0.028007 |
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Target: 5'- cGGGUGUucgUCGUCGGGGCUgaCUCCCGCUg -3' miRNA: 3'- -UCCGCG---AGCGGCCCCGGa-GAGGGCGG- -5' |
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| 31808 | 3' | -66.7 | NC_006938.1 | + | 12884 | 1.1 | 0.000099 |
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Target: 5'- cAGGCGCUCGCCGGGGCCUCUCCCGCCu -3' miRNA: 3'- -UCCGCGAGCGGCCCCGGAGAGGGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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