Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31817 | 3' | -49.7 | NC_006938.1 | + | 2491 | 0.67 | 0.955377 |
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Target: 5'- gGAGUaCUUCcuggaggcgcaGCUCGCUCGGaUCCGa -3' miRNA: 3'- gUUCAaGAAGa----------UGAGCGAGCUgAGGC- -5' |
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| 31817 | 3' | -49.7 | NC_006938.1 | + | 28582 | 0.67 | 0.955377 |
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Target: 5'- aCAAGUUCUUCgcGCUCuaucagcagGCgaUCGAcCUCCGu -3' miRNA: 3'- -GUUCAAGAAGa-UGAG---------CG--AGCU-GAGGC- -5' |
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| 31817 | 3' | -49.7 | NC_006938.1 | + | 63309 | 0.67 | 0.95495 |
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Target: 5'- -cAGUUaUUUCgauUGCUCGCUCGACaacucgaacgaggUCCGg -3' miRNA: 3'- guUCAA-GAAG---AUGAGCGAGCUG-------------AGGC- -5' |
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| 31817 | 3' | -49.7 | NC_006938.1 | + | 61036 | 0.7 | 0.859441 |
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Target: 5'- --cGUUCcUCUGCUCGCUCucaUCCa -3' miRNA: 3'- guuCAAGaAGAUGAGCGAGcugAGGc -5' |
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| 31817 | 3' | -49.7 | NC_006938.1 | + | 14720 | 0.78 | 0.437159 |
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Target: 5'- cCAAGUUCUUC-ACUCGU--GACUCCGu -3' miRNA: 3'- -GUUCAAGAAGaUGAGCGagCUGAGGC- -5' |
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| 31817 | 3' | -49.7 | NC_006938.1 | + | 17750 | 1.1 | 0.004354 |
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Target: 5'- aCAAGUUCUUCUACUCGCUCGACUCCGu -3' miRNA: 3'- -GUUCAAGAAGAUGAGCGAGCUGAGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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