Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31817 | 5' | -55.9 | NC_006938.1 | + | 36747 | 0.66 | 0.69157 |
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Target: 5'- cGGCUGGCUCGac-AGCuu-GGUCCAGg -3' miRNA: 3'- -CUGACCGAGCcucUCGucuUCAGGUU- -5' |
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| 31817 | 5' | -55.9 | NC_006938.1 | + | 28455 | 0.66 | 0.658555 |
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Target: 5'- gGGC-GGC-CGGAGGGaCAGcuGUCCAGc -3' miRNA: 3'- -CUGaCCGaGCCUCUC-GUCuuCAGGUU- -5' |
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| 31817 | 5' | -55.9 | NC_006938.1 | + | 22182 | 0.66 | 0.644162 |
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Target: 5'- cGCUGGUUCGaGuagaacuucucgaaGGAGCcGggGUCCAu -3' miRNA: 3'- cUGACCGAGC-C--------------UCUCGuCuuCAGGUu -5' |
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| 31817 | 5' | -55.9 | NC_006938.1 | + | 20333 | 0.67 | 0.613126 |
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Target: 5'- uGGCUGGCU-GGAGcugaAGCAGGuggacagcuggccGGUCCGc -3' miRNA: 3'- -CUGACCGAgCCUC----UCGUCU-------------UCAGGUu -5' |
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| 31817 | 5' | -55.9 | NC_006938.1 | + | 19254 | 0.69 | 0.474675 |
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Target: 5'- aGACUGGCUCcGAGAGgCGGAgcccGGUgaCCAGa -3' miRNA: 3'- -CUGACCGAGcCUCUC-GUCU----UCA--GGUU- -5' |
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| 31817 | 5' | -55.9 | NC_006938.1 | + | 35622 | 0.72 | 0.304471 |
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Target: 5'- cACUGcGCUCGGAG-GCcuggaAGAAGUCCGc -3' miRNA: 3'- cUGAC-CGAGCCUCuCG-----UCUUCAGGUu -5' |
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| 31817 | 5' | -55.9 | NC_006938.1 | + | 31817 | 0.73 | 0.296932 |
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Target: 5'- cGACUGGCUCGaGcugcuGGAGCGGGAGcuguUCCGu -3' miRNA: 3'- -CUGACCGAGC-C-----UCUCGUCUUC----AGGUu -5' |
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| 31817 | 5' | -55.9 | NC_006938.1 | + | 17784 | 1.06 | 0.001334 |
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Target: 5'- gGACUGGCUCGGAGAGCAGAAGUCCAAg -3' miRNA: 3'- -CUGACCGAGCCUCUCGUCUUCAGGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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