Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31818 | 5' | -60.4 | NC_006938.1 | + | 37764 | 0.66 | 0.432643 |
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Target: 5'- -aUGCCCUGAUgCUGGCcgauCGCAUCUu -3' miRNA: 3'- uaGCGGGGCUA-GACCGcc--GCGUAGAu -5' |
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| 31818 | 5' | -60.4 | NC_006938.1 | + | 52957 | 0.67 | 0.405051 |
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Target: 5'- --gGCuCCCGAg--GGUGGUGCAUCg- -3' miRNA: 3'- uagCG-GGGCUagaCCGCCGCGUAGau -5' |
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| 31818 | 5' | -60.4 | NC_006938.1 | + | 12384 | 0.67 | 0.387283 |
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Target: 5'- -gCGCCUgcgauaGAUCUGGUGGCGC-UCc- -3' miRNA: 3'- uaGCGGGg-----CUAGACCGCCGCGuAGau -5' |
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| 31818 | 5' | -60.4 | NC_006938.1 | + | 16871 | 0.67 | 0.370036 |
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Target: 5'- -gCGCCaCCGGgugagauacUUGGUGGCGCAUCc- -3' miRNA: 3'- uaGCGG-GGCUa--------GACCGCCGCGUAGau -5' |
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| 31818 | 5' | -60.4 | NC_006938.1 | + | 59510 | 0.68 | 0.353322 |
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Target: 5'- aGUCGgCCgGGUCcGGCGGCGCcacUCg- -3' miRNA: 3'- -UAGCgGGgCUAGaCCGCCGCGu--AGau -5' |
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| 31818 | 5' | -60.4 | NC_006938.1 | + | 35541 | 0.71 | 0.205036 |
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Target: 5'- --aG-CCCGAUCUGGUGGCGgAUCa- -3' miRNA: 3'- uagCgGGGCUAGACCGCCGCgUAGau -5' |
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| 31818 | 5' | -60.4 | NC_006938.1 | + | 18485 | 1.04 | 0.000712 |
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Target: 5'- cAUCGCCCCGAUCUGGCGGCGCAUCUAc -3' miRNA: 3'- -UAGCGGGGCUAGACCGCCGCGUAGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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