Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31821 | 3' | -54.8 | NC_006938.1 | + | 40954 | 0.69 | 0.578845 |
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Target: 5'- -aGGGCCGUGACaacguCCUCcGAGgUCGUg -3' miRNA: 3'- acCCUGGCACUGau---GGAG-UUCgAGCG- -5' |
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| 31821 | 3' | -54.8 | NC_006938.1 | + | 17104 | 0.7 | 0.546639 |
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Target: 5'- -uGGAUCGagGACUccGCCUCGagcAGCUUGCg -3' miRNA: 3'- acCCUGGCa-CUGA--UGGAGU---UCGAGCG- -5' |
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| 31821 | 3' | -54.8 | NC_006938.1 | + | 44591 | 0.7 | 0.545574 |
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Target: 5'- cGGGACUGUcGCUggGCCUCAcaugAGCuuccuugUCGCa -3' miRNA: 3'- aCCCUGGCAcUGA--UGGAGU----UCG-------AGCG- -5' |
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| 31821 | 3' | -54.8 | NC_006938.1 | + | 11428 | 0.71 | 0.474089 |
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Target: 5'- cGGGAacaGUGGagcCCUCGGGCUCGUc -3' miRNA: 3'- aCCCUgg-CACUgauGGAGUUCGAGCG- -5' |
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| 31821 | 3' | -54.8 | NC_006938.1 | + | 46655 | 0.72 | 0.454241 |
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Target: 5'- cGGGGCCGUGuc-GCCUCuccaGGGCUuCGUg -3' miRNA: 3'- aCCCUGGCACugaUGGAG----UUCGA-GCG- -5' |
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| 31821 | 3' | -54.8 | NC_006938.1 | + | 48891 | 0.73 | 0.39756 |
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Target: 5'- cGGGAgaGUGAUUGCCUCGuccuGCUC-Ca -3' miRNA: 3'- aCCCUggCACUGAUGGAGUu---CGAGcG- -5' |
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| 31821 | 3' | -54.8 | NC_006938.1 | + | 61741 | 0.78 | 0.203352 |
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Target: 5'- gGGGACgGcGACUACCUgGAGUUCGa -3' miRNA: 3'- aCCCUGgCaCUGAUGGAgUUCGAGCg -5' |
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| 31821 | 3' | -54.8 | NC_006938.1 | + | 19804 | 1.11 | 0.000993 |
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Target: 5'- gUGGGACCGUGACUACCUCAAGCUCGCc -3' miRNA: 3'- -ACCCUGGCACUGAUGGAGUUCGAGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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