Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31822 | 5' | -57.5 | NC_006938.1 | + | 20952 | 0.66 | 0.650456 |
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Target: 5'- -cCCaGAgccUGGAGACGCCGaGGucUCGGGc -3' miRNA: 3'- uaGGcCU---AUCUCUGCGGC-CU--AGCCCu -5' |
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| 31822 | 5' | -57.5 | NC_006938.1 | + | 34229 | 0.66 | 0.63974 |
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Target: 5'- uUCUGGAUcuGGAUGCCgucGGAUCGGc- -3' miRNA: 3'- uAGGCCUAucUCUGCGG---CCUAGCCcu -5' |
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| 31822 | 5' | -57.5 | NC_006938.1 | + | 12494 | 0.67 | 0.575618 |
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Target: 5'- -aUCGGAUAGAGA--CCGGAgagauccuggCGGGAc -3' miRNA: 3'- uaGGCCUAUCUCUgcGGCCUa---------GCCCU- -5' |
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| 31822 | 5' | -57.5 | NC_006938.1 | + | 37008 | 0.71 | 0.354944 |
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Target: 5'- uGUCCGaGGUcAGGGACaGCCGGAccggcgcgCGGGAc -3' miRNA: 3'- -UAGGC-CUA-UCUCUG-CGGCCUa-------GCCCU- -5' |
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| 31822 | 5' | -57.5 | NC_006938.1 | + | 18585 | 0.74 | 0.217095 |
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Target: 5'- cGUCCGGccgGUAGAuGCGCCGccaGAUCGGGGc -3' miRNA: 3'- -UAGGCC---UAUCUcUGCGGC---CUAGCCCU- -5' |
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| 31822 | 5' | -57.5 | NC_006938.1 | + | 20279 | 1.06 | 0.001146 |
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Target: 5'- gAUCCGGAUAGAGACGCCGGAUCGGGAc -3' miRNA: 3'- -UAGGCCUAUCUCUGCGGCCUAGCCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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