Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31823 | 5' | -54.2 | NC_006938.1 | + | 16257 | 0.66 | 0.767747 |
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Target: 5'- cCAUCCAGuG-GCACGAc--GUGCCgGAg -3' miRNA: 3'- -GUAGGUC-CaCGUGCUucuCACGGaCU- -5' |
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| 31823 | 5' | -54.2 | NC_006938.1 | + | 33672 | 0.67 | 0.736525 |
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Target: 5'- --cCCAGGUGCGCGAcacccacGAGgGCCa-- -3' miRNA: 3'- guaGGUCCACGUGCUu------CUCaCGGacu -5' |
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| 31823 | 5' | -54.2 | NC_006938.1 | + | 51926 | 0.68 | 0.671524 |
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Target: 5'- uCGUCgAuGGUGcCACGGAGAGUGgUCUGc -3' miRNA: 3'- -GUAGgU-CCAC-GUGCUUCUCAC-GGACu -5' |
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| 31823 | 5' | -54.2 | NC_006938.1 | + | 34143 | 0.69 | 0.616207 |
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Target: 5'- gAUCguGGUGCuCGGcccGGAG-GCCUGGa -3' miRNA: 3'- gUAGguCCACGuGCU---UCUCaCGGACU- -5' |
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| 31823 | 5' | -54.2 | NC_006938.1 | + | 53458 | 0.71 | 0.486848 |
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Target: 5'- aCGUCCAGGuUGUcaACGGAGAG-GCCg-- -3' miRNA: 3'- -GUAGGUCC-ACG--UGCUUCUCaCGGacu -5' |
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| 31823 | 5' | -54.2 | NC_006938.1 | + | 20795 | 0.97 | 0.010397 |
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Target: 5'- gCAUCCAGGUGCACGAGaAGUGCCUGAu -3' miRNA: 3'- -GUAGGUCCACGUGCUUcUCACGGACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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