Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31827 | 5' | -53.5 | NC_006938.1 | + | 1824 | 0.69 | 0.703967 |
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Target: 5'- gGGGCcaguugccACCGGCcGGAGCuguccaugGCG-UCGUCGc -3' miRNA: 3'- -CCCG--------UGGCCGuUCUUG--------UGCaAGCAGC- -5' |
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| 31827 | 5' | -53.5 | NC_006938.1 | + | 55979 | 0.69 | 0.693259 |
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Target: 5'- cGGGCACCuGGCcGGAGCccuCGcUCGccUCGa -3' miRNA: 3'- -CCCGUGG-CCGuUCUUGu--GCaAGC--AGC- -5' |
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| 31827 | 5' | -53.5 | NC_006938.1 | + | 22308 | 0.7 | 0.67168 |
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Target: 5'- -aGCGCUGGCuGGAGCACGUcuUCGa-- -3' miRNA: 3'- ccCGUGGCCGuUCUUGUGCA--AGCagc -5' |
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| 31827 | 5' | -53.5 | NC_006938.1 | + | 3774 | 0.7 | 0.64342 |
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Target: 5'- cGGGCGCggUGGCAAGGguggcaucgcccggcACGCGgucucgCGUCGc -3' miRNA: 3'- -CCCGUG--GCCGUUCU---------------UGUGCaa----GCAGC- -5' |
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| 31827 | 5' | -53.5 | NC_006938.1 | + | 30722 | 0.71 | 0.584698 |
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Target: 5'- aGGGUcgCGGCucGGGCGCGUUCGUg- -3' miRNA: 3'- -CCCGugGCCGuuCUUGUGCAAGCAgc -5' |
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| 31827 | 5' | -53.5 | NC_006938.1 | + | 13981 | 0.73 | 0.500088 |
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Target: 5'- uGGCGCUGGCGaauguAGAACuCGaggUCGUCGu -3' miRNA: 3'- cCCGUGGCCGU-----UCUUGuGCa--AGCAGC- -5' |
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| 31827 | 5' | -53.5 | NC_006938.1 | + | 3326 | 0.74 | 0.440335 |
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Target: 5'- aGGGCACCGGCAAGAugAagg-CGg-- -3' miRNA: 3'- -CCCGUGGCCGUUCUugUgcaaGCagc -5' |
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| 31827 | 5' | -53.5 | NC_006938.1 | + | 57007 | 0.75 | 0.384854 |
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Target: 5'- -aGCAUCGGCGgucagcAGGGCGCGUUUGUCu -3' miRNA: 3'- ccCGUGGCCGU------UCUUGUGCAAGCAGc -5' |
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| 31827 | 5' | -53.5 | NC_006938.1 | + | 48351 | 0.76 | 0.350529 |
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Target: 5'- cGGGCGgCGGUGAcGAGCGCGUccUGUCGg -3' miRNA: 3'- -CCCGUgGCCGUU-CUUGUGCAa-GCAGC- -5' |
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| 31827 | 5' | -53.5 | NC_006938.1 | + | 22446 | 1.12 | 0.001259 |
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Target: 5'- aGGGCACCGGCAAGAACACGUUCGUCGg -3' miRNA: 3'- -CCCGUGGCCGUUCUUGUGCAAGCAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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