Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31830 | 3' | -55.2 | NC_006938.1 | + | 5264 | 0.71 | 0.481633 |
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Target: 5'- gGCCUGUCCa-GugGCCGAGggGCGAg -3' miRNA: 3'- aCGGGCAGGagUugUGGCUCa-UGCUg -5' |
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| 31830 | 3' | -55.2 | NC_006938.1 | + | 11181 | 0.72 | 0.404593 |
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Target: 5'- cGCCauccUCCUCGACGCCGAGgccugUGGCa -3' miRNA: 3'- aCGGgc--AGGAGUUGUGGCUCau---GCUG- -5' |
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| 31830 | 3' | -55.2 | NC_006938.1 | + | 13272 | 0.72 | 0.395519 |
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Target: 5'- cGCCUGUCCcacucccggUggUGCCGGGUGCGAa -3' miRNA: 3'- aCGGGCAGGa--------GuuGUGGCUCAUGCUg -5' |
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| 31830 | 3' | -55.2 | NC_006938.1 | + | 42495 | 0.72 | 0.394619 |
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Target: 5'- gGCCgCGUCggcgaagaaCUCGACGCCGAggaagcaGUGCGGCu -3' miRNA: 3'- aCGG-GCAG---------GAGUUGUGGCU-------CAUGCUG- -5' |
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| 31830 | 3' | -55.2 | NC_006938.1 | + | 6311 | 0.74 | 0.307747 |
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Target: 5'- cUGUCCGgccuucgccuuguucUCCUCGACcagcuCCGAGUugGACu -3' miRNA: 3'- -ACGGGC---------------AGGAGUUGu----GGCUCAugCUG- -5' |
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| 31830 | 3' | -55.2 | NC_006938.1 | + | 50664 | 0.77 | 0.218571 |
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Target: 5'- cGCCCGUCuCUucgaaacCGAC-CCGAGUGCGAUu -3' miRNA: 3'- aCGGGCAG-GA-------GUUGuGGCUCAUGCUG- -5' |
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| 31830 | 3' | -55.2 | NC_006938.1 | + | 24187 | 1.1 | 0.001004 |
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Target: 5'- gUGCCCGUCCUCAACACCGAGUACGACg -3' miRNA: 3'- -ACGGGCAGGAGUUGUGGCUCAUGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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